All tags RabMAb High affinity: the fourth RabMAb advantage

High affinity: the fourth RabMAb advantage

Antibody affinity is typically represented by the equilibrium dissociation constant (KD), a ratio of koff/kon between the antibody and its antigen, where lower KD value suggests higher affinity relationship (1). While most therapeutic monoclonal antibodies have a KD value in the nanomolar range (KD = 10-9 M), rabbit monoclonals consistently demonstrate higher affinity, with the KD values which can often reach the picomolar level (KD = 10-12 M), effectively eliminating the need for further affinity maturation (2).

In terms of your experiment, a high affinity means that you can use more stringent conditions without worrying about the loss of signal strength, therefore ensuring the cleanest results with the highest possible specificity.

Affinity comparison by KD value for antibodies generated with RabMAb® ​technology vs. popular therapeutic antibodies

RabMAbKD (M)
ER1.28 x 10-12
ID12.82 x 10-12
C299.57 x 10-11
TNF-alpha1.25 x 10-11
IL-1-beta1.99 x 10-10
Therapeutic MAbKD (M)
Herceptin5.0 x 10-9
Rituxan8.0 x 10-9
Synagis1.0 x 10-9
Remicade2.0 x 10-10
Avastin5.0 x 10-10
















Affinity meter: RabMAb primary antibodies vs. typical monoclonal antibodies

Affinity meter

Antibody affinity comparison by KD value for RabMAb primary antibodies vs. typical monoclonal antibodies. Measurement based on KD measurement analysis of over 850 RabMAb primaries. RabMAb primary antibodies display antibody affinity level in the picomolar range compared to typical MAbs which are in the nano molecular range.

References

  • 1. Applications And Engineering Of Monoclonal Antibodies. David J. King, 2007.
  • 2. Anti-peptide antibody screening: Selection of high affinity monoclonal reagents by a refined surface plasmon resonance technique. Pope ME, [J Immunol Methods. 2009 Feb 28;341(1-2):86-96. doi: 10.1016/j.jim.2008.11.004. Epub 2008 Nov 28]