All tags Primary antibodies Endothelial cell markers

Endothelial cell markers

Quickly select endothelial cell markers based on your application needs.

We've put together a list of widely used endothelial cell markers and which applications these have been tested in  – making it easier and quicker for you to choose the best tools for your experiment. 


Protein

Localization

Antibody

Clonality

WB

IP

IHC

ICC

Flow

CD31

Endothelial cells, B cells, platelets, macrophages, monocytes, NK cells, T cells1

ab28364  

Poly



RabMAb® ab76533

Mono


*

CD34

Dendritic cells, HSCs, leukemic cells, endothelial progenitor cells, endothelial cells of blood vessels2

RabMAb® ab81289

Mono


ab8536

Mono


*

CD45

Hematopoietic cells (except mature erythrocytes)3

RabMAb® ab40763

Mono


*

ab8216

Mono

*

ICAM-1/
CD54

Vascular endothelium, lymphocytes, macrophages4


RabMAb® ab53013

Mono


*

 ab2213

Mono

*

Protein

Localization

Antibody

Clonality

WB

IP

IHC

ICC

Flow

LYVE-1

Lymphatic endothelial cells5

 ab14917

Poly


RabMAb® ab183501

Mono





Tie-2/
Tek

Vascular endothelial cells6

ab24859

Mono



*

RabMAb®
ab151704

Mono




VCAM-1/ CD106

Low expression on large proportion of resting vascular endothelial cells – inducible following injury7

RabMAb® ab134047

Mono


ab7224

Mono


*

VE cadherin

Junctions between endothelial cells and on HSCs and mesenchymal stem cells8

 ab33168

Poly

*

 RabMAb® ab205336

Mono



Protein

Localization

Antibody

Clonality

WB

IP

IHC

ICC

Flow

VEGF-R2

Endothelial cells, endothelial cell progenitors9


ab39256

Poly



ab9530

Mono



von Willebrand factor (vWF)

Endothelial cells megakaryocytes10

ab6994

Poly


RabMAb® ab179451

Mono




*♦ Click on the links in the ‘Flow’ column for the conjugated versions of these antibodies.

WB = wester blot, IP = immunoprecipitation, IHC = immunohistochemistry, ICC = immunocytochemistry, Flow = flow cytometry, HSC = hematopoietic stem cell, Poly = polyclonal antibody, Mono = monoclonal antibody

References

1.  Nourshargh, S. The role of JAM-A and PECAM-1 in modulating leukocyte infiltration in inflamed and ischemic tissues. J. Leukoc. Biol. 80, 714–718 (2006).

2. Sidney, L. E., Branch, M. J., Dunphy, S. E., Dua, H. S. & Hopkinson, A. Concise review: Evidence for CD34 as a common marker for diverse progenitors. Stem Cells 32, 1380–1389 (2014).

3. Nakano, A., Harada, T., Morikawa, S. & Kato, Y. Expression of leukocyte common antigen (CD45) on various human leukemia/lymphoma cell lines. Acta Pathol. Jpn. 40, 107–115 (1990).

4. Dustin, M. L., Rothlein, R., Bhan, A. K., Dinarello, C. A. & Springer, T. A. Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). J. Immunol. 137, 245–54 (1986).

5. Jackson, D. G. The lymphatics revisited: New perspectives from the hyaluronan receptor LYVE-1. Trends in Cardiovascular Medicine 13, 1–7 (2003).

6. Sato, T. N. et al. Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation. Nature 376, 70–4 (1995).

7. Ulyanova, T. et al. VCAM-1 expression in adult hematopoietic and nonhematopoietic cells is controlled by tissue-inductive signals and reflects their developmental origin. Blood 106, 86–94 (2005).

8. Vestweber, D. VE-cadherin: The major endothelial adhesion molecule controlling cellular junctions and blood vessel formation. Arteriosclerosis, Thrombosis, and Vascular Biology 28, 223–232 (2008).

9. Ferrara, N., Gerber, H. P. & LeCouter, J. The biology of VEGF and its receptors. Nat Med 9, 669–676 (2003).

10. Sadler, J. E. Biochemistry and genetics of von Willebrand factor. Annu. Rev. Biochem. 67, 395–424 (1998).


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