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Protein cleavage and proteolysis play major roles in diverse biological processes including:
Normal protein processing and maturation
Site-specific cleavage often results in protein fragments that possess distinct functional properties. The study of protein cleavage is greatly enhanced by cleavage specific antibodies that offer a simple and sensitive way to detect and quantify the cleaved fragments.
Rabbits have a unique immune system which allows them to develop antibodies that recognize subtle changes in epitopes such as those created upon protein cleavage. The high specificity and affinity of RabMAb® advantages allow unequivocal resolution of parental and cleaved protein fragments in Western blot, FACS, and IHC applications.
Our Custom Services team has a demonstrated record of success developing custom RabMAb primary antibodies that can differentiate between subtle structural variations. An example is documented in this reference, which describes anti-Amyloid beta (Abeta40 and Abeta42) antibodies developed using our RabMAb technology.
A comparison of specificity of various PARP RabMAb primary antibodies
Each Poly (ADP-ribose) polymerase (PARP-1) specific RabMAb primary antibody (ab32064, ab32561 and ab32138) recognizes one cleavage form with no cross-reactivity.
Anti-Caspase-3 RabMAb primary antibodies
One RabMAb primary antibody (ab32499) only detects pro-form of Caspase 3 and does not recognize any cleaved caspases, while the other (ab32042) only detects the active, cleaved form of Caspase 3 and does not recognize the pro-form.