This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Prickle抗体(ab15577)

概述

  • 产品名称Anti-Prickle抗体
    参阅全部 Prickle 一抗
  • 描述
    兔多克隆抗体to Prickle

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • 特异性Immunogen sequence most homologous with Prickle 1, but is ~65% identical with Prickle 2 sequences, thus some reaction with Prickle 2 may be seen as well as with Prickle 1.
  • 种属反应性

    预测可用于: Mouse, Rat, Human, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Xenopus laevis Prickle.

    (Peptide available as ab15578.)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration. This antibody gave a positive result in ELISA against the immunizing peptide (ab15578).

靶标

Anti-Prickle antibody 图像

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • ab15577 (1/50) staining Prickle in paraffin-embbeded human uterus (smooth muscle). Tissues underwent formaldehyde fixation, heat mediated antigen retrieval and peroxidase blocking prior to staining of the sections. Please refer to Abreview for further experimental details.

    See Abreview

Anti-Prickle antibody (ab15577)参考文献

ab15577 has not yet been referenced specifically in any publications.

Product Wall

This antibody was raised against Prickle1.

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Uterus (smooth muscle))
Specification Uterus (smooth muscle)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Feb 01 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Bone and muscle)
Specification Bone and muscle
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 09 2008

Thank you for your enquiry. I have performed an alignment of your ascidian prickle sequence against our immunogen sequence. The alignment can be accessed at the following ClustalW link for the next 24 hours. http://www.ebi.ac.uk/cgi-bin/jobresu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"