Anti-PRG2抗体[1.B.787] (ab14462)

概述

  • 产品名称Anti-PRG2抗体[1.B.787]
    参阅全部 PRG2 一抗
  • 描述
    小鼠单克隆抗体[1.B.787] to PRG2
  • 特异性Specific for eosinophil major basic protein (14kDa) irrespective of the stages of eosinophil activation and can be regarded as a “pan-eosinophil” marker. Recognizes secreted pro-form of MBP (Pregnancy associated MBP, 23.8kDa) and mature form found in the matrix of the eosinophil large specific granule (13.8kDa).
  • 经测试应用适用于: ELISA, IHC-Fr, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat, Horse
  • 免疫原

    Full length protein (Human).

性能

应用

Our Abpromise guarantee covers the use of ab14462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
IHC-Fr
IHC-P
WB
  • 应用说明ELISA: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol (0.1% trypsin).
    IHC-Fr: Use at an assay dependent dilution. Fix with methanol:acetone.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 26.6 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
    • 组织特异性High levels of the proform in placenta and pregnancy serum; in placenta, localized to X cells of septa and anchoring villi. Lower levels in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow and colon.
    • 序列相似性Contains 1 C-type lectin domain.
    • 发展阶段Levels of the proform increase in serum and placenta during pregnancy.
    • 翻译后修饰Nitrated.
    • 细胞定位Cytoplasmic vesicle > secretory vesicle. The proform is secreted. The mature protein is found in the matrix of the eosinophil's large specific granule and Secreted. The proform is secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BMPG antibody
      • EMBP antibody
      • Eosinophil granule major basic protein antibody
      • Eosinophil major basic protein antibody
      • MBP antibody
      • MBP1 antibody
      • Natural killer cell activator antibody
      • Pregnancy-associated major basic protein antibody
      • PRG 2 antibody
      • PRG2 antibody
      • PRG2_HUMAN antibody
      • Proteoglycan 2 antibody
      • Proteoglycan 2 bone marrow antibody
      • Proteoglycan 2 preproprotein antibody
      see all

    Anti-PRG2 antibody [1.B.787] (ab14462)参考文献

    ab14462 has not yet been referenced specifically in any publications.

    Product Wall

    I have received confirmation from the laboratory that tested ab14462 that your protocol is very good and should work with ab14462. The laboratory shared also the following feedback which may interest you "Your customer's secondary antibody is a She...

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    I have been informed by the source of ab14462 that it was not tested in house in ELISA and that unfortunately we therefore do not know the recommended dilution. I was recommended to start at a concentration of 2-5ug/ml (for Direct or Indirect ELISA), ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"