RabMAb

Anti-Presenilin 2抗体[EP1515Y] (ab51249)

概述

  • 产品名称
    Anti-Presenilin 2抗体[EP1515Y]
    参阅全部 Presenilin 2 一抗
  • 描述
    兔单克隆抗体[EP1515Y] to Presenilin 2
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues on the C terminus of human Presenilin 2.

  • 阳性对照
    • WB: PC-12 and HeLa cell lysate. IHC-P: Human kidney tissue. ICC/IF: HepG2, JAR and A-673 cells.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab51249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/20000. Detects a band of approximately 23 kDa (predicted molecular weight: 50 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
IP 1/50.
  • 应用说明
    Is unsuitable for Flow Cyt.
  • 靶标

    • 功能
      Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins.
    • 组织特异性
      Isoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.
    • 疾病相关
      Defects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
      Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • 序列相似性
      Belongs to the peptidase A22A family.
    • 结构域
      The PAL motif is required for normal active site conformation.
    • 翻译后修饰
      Heterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
      Phosphorylated on serine residues.
    • 细胞定位
      Endoplasmic reticulum membrane. Golgi apparatus membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AD3L antibody
      • AD3LP antibody
      • AD4 antibody
      • AD5 antibody
      • Alzheimer disease 4 antibody
      • CMD1V antibody
      • E5-1 antibody
      • OTTHUMP00000035671 antibody
      • OTTHUMP00000035672 antibody
      • OTTHUMP00000228286 antibody
      • OTTHUMP00000228288 antibody
      • Presenilin 2 (Alzheimer disease 4) antibody
      • Presenilin 2 antibody
      • Presenilin-2 CTF subunit antibody
      • PS-2 antibody
      • PS2 antibody
      • Psen2 antibody
      • PSN2_HUMAN antibody
      • PSNL2 antibody
      • STM-2 antibody
      • STM2 antibody
      see all

    图片

    • Anti-Presenilin 2 antibody [EP1515Y] (ab51249) at 1/1000 dilution (for 16 hours at 4°C) + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

      Secondary
      HRP-conjugated Goat anti-rabbit IgG at 1/2000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 50 kDa
      Observed band size : 24 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      This image is courtesy of an anonymous Abreview

      Blocking Step: 5% Milk for 1 hour at 24°C

      See Abreview

    • Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A-673 (Human muscle Ewing's Sarcoma cell line) cells labeling Presenilin with ab51249 at 1/250 dilution, followed by Anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining on A-673 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution (red).
      The negative controls are as follows:
      -ve control 1: ab51249 at 1/250 dilution followed by Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution.

       

    • Presenilin was immunoprecipitated from 1 mg of HeLa whole cell extract with ab51249 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab51249 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Input: HeLa whole cell extract 10 µg.

       IP (+): ab51249 IP in HeLa whole cell extract.

       IP (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in HeLa whole cell extract.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

    • Anti-Presenilin 2 antibody [EP1515Y] (ab51249) at 1/200000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 50 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    • ab51249 at a 1/50 dilution staining Presenilin 2 in human kidney tissue by immunohistochemistry using paraffin embedded tissue.

    • Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized JAR (human placenta choriocarcinoma cell line) cells labeling Presenilin with ab51249 at 1/250 dilution, followed by Anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasm staining on JAR cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution (red).
      The negative controls are as follows:
      -ve control 1: ab51249 at 1/250 dilution followed by Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution.

    • Presenilin was immunoprecipitated from 1 mg of PC-12 (rat adrenal gland pheochromocytoma) whole cell extract with ab51249 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab51249 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Input: PC-12 whole cell extract 10 µg.

      IP (+): ab51249 IP in PC-12 whole cell extract.

      IP (-): Rabbit monoclonal IgG (ab172730) instead of ab51249 in PC-12 whole cell extract.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

    文献

    This product has been referenced in:
    • Wang DB  et al. Loss of endophilin-B1 exacerbates Alzheimer's disease pathology. Brain 138:2005-19 (2015). WB ; Mouse . Read more (PubMed: 25981964) »
    • Ben-Gedalya T  et al. Alzheimer's disease-causing proline substitutions lead to presenilin 1 aggregation and malfunction. EMBO J 34:2820-39 (2015). WB . Read more (PubMed: 26438723) »

    See all 6 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate, 0.05% Tween 20, pH 6.0
    Permeabilization
    No
    Specification
    brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Username

    Sulieman Minhas

    Verified customer

    提交于 Dec 13 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human iPS cell derived neurons (healthy/diseased))
    Gel Running Conditions
    Reduced Denaturing (12.5% SDS gel)
    Loading amount
    10 µg
    Specification
    human iPS cell derived neurons (healthy/diseased)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 25 2016

    Thank you for contacting us.

    The immunogen is located within residues 300 - 350 for this antibody.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    <...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (WT AND KO MEF)
    Loading amount
    30 µg
    Specification
    WT AND KO MEF
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 08 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HELA)
    Total protein in input
    200 µg
    Specification
    HELA
    Immuno-precipitation step
    Protein G
    Username

    Abcam user community

    Verified customer

    提交于 Sep 22 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton-X-0.5%
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 14 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HELA)
    Loading amount
    30 µg
    Specification
    HELA
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 30 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册