概述

  • 产品名称Anti-Presenilin 2抗体[APS 26]
    参阅全部 Presenilin 2 一抗
  • 描述
    小鼠单克隆抗体[APS 26] to Presenilin 2
  • 特异性No cross-reactivity is seen with presenilin 1.
  • 经测试应用适用于: ICC/IF, IP, ICC, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Pig, Non Human Primates
  • 免疫原

    Synthetic peptide corresponding to Human Presenilin 2 aa 317-334.
    Sequence:

    LPYDPEMEEDSYDSFGEP

  • 阳性对照
    • RAW cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab15549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21163940
IP Use at an assay dependent concentration. PubMed: 21163940
ICC Use at an assay dependent concentration.
IHC-P 1/20.
WB Use a concentration of 12 µg/ml. Detects a band of approximately 20, 45 kDa.
ELISA Use a concentration of 4.5 µg/ml.

靶标

  • 功能Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins.
  • 组织特异性Isoform 1 is seen in the placenta, skeletal muscle and heart while isoform 2 is seen in the heart, brain, placenta, liver, skeletal muscle and kidney.
  • 疾病相关Defects in PSEN2 are the cause of Alzheimer disease type 4 (AD4) [MIM:606889]. AD is an autosomal dominant Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
    Defects in PSEN2 are the cause of cardiomyopathy dilated type 1V (CMD1V) [MIM:613697]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • 序列相似性Belongs to the peptidase A22A family.
  • 结构域The PAL motif is required for normal active site conformation.
  • 翻译后修饰Heterogeneous proteolytic processing generates N-terminal and C-terminal fragments.
    Phosphorylated on serine residues.
  • 细胞定位Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AD3L antibody
    • AD3LP antibody
    • AD4 antibody
    • AD5 antibody
    • Alzheimer disease 4 antibody
    • CMD1V antibody
    • E5-1 antibody
    • OTTHUMP00000035671 antibody
    • OTTHUMP00000035672 antibody
    • OTTHUMP00000228286 antibody
    • OTTHUMP00000228288 antibody
    • Presenilin 2 (Alzheimer disease 4) antibody
    • Presenilin 2 antibody
    • Presenilin-2 CTF subunit antibody
    • PS-2 antibody
    • PS2 antibody
    • Psen2 antibody
    • PSN2_HUMAN antibody
    • PSNL2 antibody
    • STM-2 antibody
    • STM2 antibody
    see all

Anti-Presenilin 2 antibody [APS 26] 图像

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Presenilin 2 (green) with ab15549 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of A2058 cells labeling Presenilin 2 (green) with ab15549 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • IF staining Presenilin 2 in Mouse fibroblasts using ab15549 

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 2 ab15549 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Anti-Presenilin 2 antibody [APS 26] (ab15549)参考文献

This product has been referenced in:
  • Rivabene R  et al. Thapsigargin affects presenilin-2 but not presenilin-1 regulation in SK-N-BE cells. Exp Biol Med (Maywood) 239:213-24 (2014). Human . Read more (PubMed: 24363250) »
  • Soler-López M  et al. Interactome mapping suggests new mechanistic details underlying Alzheimer's disease. Genome Res 21:364-76 (2011). WB, ICC/IF, IP ; Human . Read more (PubMed: 21163940) »

See all 2 Publications for this product

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