Anti-Presenilin 1 (phospho S357)抗体(ab78914)


  • 产品名称Anti-Presenilin 1 (phospho S357)抗体
    参阅全部 Presenilin 1 一抗
  • 描述
    兔多克隆抗体to Presenilin 1 (phospho S357)
  • 特异性ab78914 detects endogenous levels of Presenilin 1 only when phosphorylated at serine 357 (Human: Ser357; Mouse: Ser357; Rat: Ser358).
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic phosphopeptide derived from human Presenilin 1 around the phosphorylation site of serine 357 (P-E-SP-R-A).

  • 阳性对照
    • Human brain tissue; RAW264.7 cell extracts, treated with UV (5mins).



Our Abpromise guarantee covers the use of ab78914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 53 kDa.
IHC-P 1/50 - 1/100.


  • 功能Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis.
  • 组织特异性Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes.
  • 疾病相关Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3) [MIM:607822]. AD3 is a familial early-onset form of Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
    Defects in PSEN1 are a cause of frontotemporal dementia [MIM:600274].
    Defects in PSEN1 are the cause of cardiomyopathy dilated type 1U (CMD1U) [MIM:613694]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in PSEN1 are the cause of acne inversa familial type 3 (ACNIF3) [MIM:613737]. A chronic relapsing inflammatory disease of the hair follicles characterized by recurrent draining sinuses, painful skin abscesses, and disfiguring scars. Manifestations typically appear after puberty.
  • 序列相似性Belongs to the peptidase A22A family.
  • 结构域The PAL motif is required for normal active site conformation.
  • 翻译后修饰Heterogeneous proteolytic processing generates N-terminal (NTF) and C-terminal (CTF) fragments of approximately 35 and 20 kDa, respectively. During apoptosis, the C-terminal fragment (CTF) is further cleaved by caspase-3 to produce the fragment, PS1-CTF12.
    After endoproteolysis, the C-terminal fragment (CTF) is phosphorylated on serine residues by PKA and/or PKC. Phosphorylation on Ser-346 inhibits endoproteolysis.
  • 细胞定位Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell surface. Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane. Also present in azurophil granules of neutrophils.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AD3 antibody
    • Ad3h antibody
    • FAD antibody
    • Homo Sapiens Clone CC44 Senilin 1 antibody
    • Presenilin-1 CTF12 antibody
    • Protein S182 antibody
    • PS 1 antibody
    • PS-1 antibody
    • PS1-CTF12 antibody
    • PSEN1 antibody
    • PSN1_HUMAN antibody
    • PSNL1 antibody
    • S182 antibody
    see all

Anti-Presenilin 1 (phospho S357) antibody 图像

  • ab78914 at 1/50 dilution staining Presenilin 1 in human brain by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
  • All lanes : Anti-Presenilin 1 (phospho S357) antibody (ab78914) at 1/500 dilution

    Lane 1 : RAW264.7 cell extracts, treated with UV (5mins)
    Lane 2 : RAW264.7 cell extracts, treated with UV (5mins) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 53 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)

Anti-Presenilin 1 (phospho S357) antibody (ab78914)参考文献

ab78914 has not yet been referenced specifically in any publications.

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