Anti-Presenilin 1抗体[APS 11] (ab15456)

概述

  • 产品名称Anti-Presenilin 1抗体[APS 11]
    参阅全部 Presenilin 1 一抗
  • 描述
    小鼠单克隆抗体[APS 11] to Presenilin 1
  • 特异性No cross-reactivity is seen with presenilin 2. In formalin-fixed, paraffin embedded sections of human brain, this antibody showed strong staining of both the plaque core and dystrophic neurites. By Western blot, this antibody detects an ~28 kDa protein representing PS1 N-terminus cleavage product in ST15 cell lysate transfected with human PS1.
  • 经测试应用适用于: IP, IHC-FoFr, ICC, ICC/IF, WB, ELISA, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog, Non Human Primates, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide corresponding to Human Presenilin 1 aa 21-34.
    Sequence:

    HLSNTVRSQNDNRE

  • 阳性对照
    • IHC: Human brain tissue slides. WB: ST15 cell lysate transfected with human PS1.

性能

应用

Our Abpromise guarantee covers the use of ab15456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. PubMed: 21163940
IHC-FoFr Use at an assay dependent concentration. PubMed: 18927449
ICC Use at an assay dependent concentration.
ICC/IF Use a concentration of 0.75 µg/ml.
WB Use a concentration of 12 µg/ml. Detects a band of approximately 28 kDa.
ELISA Use a concentration of 4.5 µg/ml.
IHC-P Use a concentration of 0.75 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis.
  • 组织特异性Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes.
  • 疾病相关Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3) [MIM:607822]. AD3 is a familial early-onset form of Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
    Defects in PSEN1 are a cause of frontotemporal dementia [MIM:600274].
    Defects in PSEN1 are the cause of cardiomyopathy dilated type 1U (CMD1U) [MIM:613694]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in PSEN1 are the cause of acne inversa familial type 3 (ACNIF3) [MIM:613737]. A chronic relapsing inflammatory disease of the hair follicles characterized by recurrent draining sinuses, painful skin abscesses, and disfiguring scars. Manifestations typically appear after puberty.
  • 序列相似性Belongs to the peptidase A22A family.
  • 结构域The PAL motif is required for normal active site conformation.
  • 翻译后修饰Heterogeneous proteolytic processing generates N-terminal (NTF) and C-terminal (CTF) fragments of approximately 35 and 20 kDa, respectively. During apoptosis, the C-terminal fragment (CTF) is further cleaved by caspase-3 to produce the fragment, PS1-CTF12.
    After endoproteolysis, the C-terminal fragment (CTF) is phosphorylated on serine residues by PKA and/or PKC. Phosphorylation on Ser-346 inhibits endoproteolysis.
  • 细胞定位Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell surface. Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane. Also present in azurophil granules of neutrophils.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AD3 antibody
    • Ad3h antibody
    • FAD antibody
    • Homo Sapiens Clone CC44 Senilin 1 antibody
    • Presenilin-1 CTF12 antibody
    • Protein S182 antibody
    • PS 1 antibody
    • PS-1 antibody
    • PS1-CTF12 antibody
    • PSEN1 antibody
    • PSN1_HUMAN antibody
    • PSNL1 antibody
    • S182 antibody
    see all

Anti-Presenilin 1 antibody [APS 11] 图像

  • IF staining PS1 using ab15456.
  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 ab15456 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 ab15456 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Overlay histogram showing HepG2 cells stained with ab15456 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab15456, 1ug/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG1 (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Presenilin 1 antibody [APS 11] (ab15456)参考文献

This product has been referenced in:
  • Bakele M  et al. An Interactive Network of Elastase, Secretases, and PAR-2 Protein Regulates CXCR1 Receptor Surface Expression on Neutrophils. J Biol Chem 289:20516-20525 (2014). IP, ICC/IF ; Human . Read more (PubMed: 24914212) »
  • Suzuki T  et al. Cell type-specific subcellular localization of phospho-TBK1 in response to cytoplasmic viral DNA. PLoS One 8:e83639 (2013). ICC/IF ; Human . Read more (PubMed: 24349538) »

See all 6 Publications for this product

Product Wall

Thank you for your reply.


Based on the information you have provided, I would recommend increasing the amount of antibody used in the WB. The datasheet calls for 12ug/ml for WB and you have only used up to 4ug/ml. I would recommend incr...

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According to our records, ab15456 was proving difficult to use in WB and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand...

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Thank you for contacting Abcam regarding ab15456.


I am sorry that you are experiencing difficulties with this antibody in WB. I have checked our available references, and unfortunately none specifically use rat brains as samples.
<...

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Thank you for your enquiry. I'm afraid we do not have the immunizing peptide to use for blocking control experiments for ab15456. I'm very sorry I cannot help you more,

Let's see - we have the following PS1 antibodies that have been tested for cross-reactivity with human, mouse, and rat and tested in Western blotting: ab15456 (which you tried), ab15458, ab16244 (a new chicken polyclonal), and ab10281 (tested in human,...

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Thank you for your email and I'm sorry to hear that you are still experiencing difficulty with ab15456. I'm replying to you on behalf of Sarah, who is away from the office this week. As Sarah mentioned, we can send you a replacement antibody or offe...

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I'm very sorry you have a problem with ab15456, thank you for taking the time to answer our questionnaire, it is very useful. I would like to suggest the following modifications to your protocol: -use the recommended positive control of human b...

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