ab114057 is expected to recognize all three reported isoforms (NP_056202.2; NP_955776.1; NP_001094289.1).
Mouse, Rat, Human
Chicken, Cow, Dog, Pig, Xenopus laevis
Synthetic peptide corresponding to Human PREI3 aa 137-151 (internal sequence).
Human Brain (Frontal Cortex), Mouse and Rat Brain lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
Immunogen affinity purified
ab114057 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Antibody detection limit dilution 1:4,000.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Belongs to the MOB1/phocein family.
Phosphorylated on serine residues.
Cytoplasm > perinuclear region. Membrane. Golgi apparatus > Golgi stack membrane. In a perinuclear punctate pattern. Associated with membranes and the Golgi stacks.
Information by UniProt
CGI 95 antibody
class II mMOB1 antibody
Western blot - Anti-PREI3 antibody (ab114057)
All lanes : Anti-PREI3 antibody (ab114057) at 0.3 µg/ml Lane 1 : Mouse Brain lysate Lane 2 : Rat Brain lysate Lysates/proteins at 35 µg per lane. Predicted band size : 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREI3 antibody (ab114057)
ab114057 (5µg/ml) shows staining of PREI3 in paraffin embedded Human Cerebral Cortex following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows cytoplasm staining in the neuronal bodies.
Western blot - PREI3 antibody (ab114057)
Anti-PREI3 antibody (ab114057) at 0.3 µg/ml + Human Brain, Frontal Cortex lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique Predicted band size : 26 kDa Primary incubation was 1 hour.
has not yet been referenced specifically in any publications.
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