产品名称 Anti-PREI3抗体 参阅全部 PREI3 一抗
特异性 ab114057 is expected to recognize all three reported isoforms (NP_056202.2; NP_955776.1; NP_001094289.1).
ELISA, WB, IHC-P more details
Mouse, Rat, Human
Chicken, Cow, Dog, Pig, Xenopus laevis
Synthetic peptide corresponding to Human PREI3 aa 137-151 (internal sequence).
Database link: Q9Y3A3
Human Brain (Frontal Cortex), Mouse and Rat Brain lysates.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 pH: 7.30 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 ab114057 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Antibody detection limit dilution 1:4,000.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
序列相似性 Belongs to the MOB1/phocein family.
翻译后修饰 Phosphorylated on serine residues.
细胞定位 Cytoplasm > perinuclear region. Membrane. Golgi apparatus > Golgi stack membrane. In a perinuclear punctate pattern. Associated with membranes and the Golgi stacks.
Information by UniProt
CGI 95 antibody
class II mMOB1 antibody
Anti-PREI3 antibody 图像
Western blot - Anti-PREI3 antibody (ab114057)
All lanes : Anti-PREI3 antibody (ab114057) at 0.3 µg/ml Lane 1 : Mouse Brain lysate Lane 2 : Rat Brain lysate Lysates/proteins at 35 µg per lane. Predicted band size : 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREI3 antibody (ab114057)
ab114057 (5µg/ml) shows staining of PREI3 in paraffin embedded Human Cerebral Cortex following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows cytoplasm staining in the neuronal bodies.
Western blot - PREI3 antibody (ab114057)
Anti-PREI3 antibody (ab114057) at 0.3 µg/ml + Human Brain, Frontal Cortex lysate (in RIPA buffer) at 35 µg developed using the ECL technique Predicted band size : 26 kDa Primary incubation was 1 hour.
Anti-PREI3 antibody (ab114057)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"