概述

  • 产品名称Anti-PREI3抗体
    参阅全部 PREI3 一抗
  • 描述
    山羊多克隆抗体to PREI3
  • 特异性ab114057 is expected to recognize all three reported isoforms (NP_056202.2; NP_955776.1; NP_001094289.1).
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow, Dog, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human PREI3 aa 137-151 (internal sequence).
    Sequence:

    C-NSNKYFPSRVSIKES


    Database link: Q9Y3A3

  • 阳性对照
    • Human Brain (Frontal Cortex), Mouse and Rat Brain lysates.

性能

应用

Our Abpromise guarantee covers the use of ab114057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:4,000.

WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-PREI3 antibody 图像

  • All lanes : Anti-PREI3 antibody (ab114057) at 0.3 µg/ml

    Lane 1 : Mouse Brain lysate
    Lane 2 : Rat Brain lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 26 kDa
  • ab114057 (5µg/ml) shows staining of PREI3 in paraffin embedded Human Cerebral Cortex following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows cytoplasm staining in the neuronal bodies.

  • Anti-PREI3 antibody (ab114057) at 0.3 µg/ml + Human Brain, Frontal Cortex lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 26 kDa
    Primary incubation was 1 hour.

Anti-PREI3 antibody (ab114057)参考文献

ab114057 has not yet been referenced specifically in any publications.

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