Recombinant Protein Epitope Signature Tag antigen: QFSINTTSIS VNKLFVRVFT VHPNLCFHYS WVAEDHQGAQ HTANRVFSLS GSYIHLEPVA GTLPCGHTET ITAHYT, corresponding to amino acids 402 - 477 of Human Pregnancy zone protein. (P20742).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.