概述

  • 产品名称Anti-PREB抗体
    参阅全部 PREB 一抗
  • 描述
    兔多克隆抗体to PREB
  • 特异性This antibody is specific for PREB.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A region within synthetic peptide: RVENLQAVQT DFSSDPLQKV VCFNHDNTLL ATGGTDGYVR VWKVPSLEKV, corresponding to amino acids 140-189 of Human PREB

  • 阳性对照
    • Raji cell lysate and human heart tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab42501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 41, 30 kDa (predicted molecular weight: 45 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription (By similarity). Guanine nucleotide exchange factor that activates SARA2. Required for the formation of COPII transport vesicles from the ER.
  • 组织特异性Ubiquitous.
  • 序列相似性Contains 3 WD repeats.
  • 细胞定位Nucleus. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Mammalian guanine nucleotide exchange factor mSec12 antibody
    • Preb antibody
    • PREB_HUMAN antibody
    • Prolactin regulatory binding element protein antibody
    • Prolactin regulatory element binding antibody
    • Prolactin regulatory element binding protein antibody
    • Prolactin regulatory element-binding protein antibody
    • SEC12 antibody
    see all

Anti-PREB antibody 图像

  • Anti-PREB antibody (ab42501) at 0.5 µg/ml + Raji cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 41& 30 kDa (why is the actual band size different from the predicted?)
  • ab42501 at 4µg/ml staining PREB in human myocardial cells.

Anti-PREB antibody (ab42501)参考文献

ab42501 has not yet been referenced specifically in any publications.

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