Anti-PREB抗体(ab3422)

概述

  • 产品名称Anti-PREB抗体
    参阅全部 PREB 一抗
  • 描述
    兔多克隆抗体to PREB
  • 特异性Detects recombinant mouse PREB (Prolactin Regulatory Element Binding protein).
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant fragment corresponding to Mouse PREB.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Constituent: 99% PBS
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

靶标

  • 功能Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription (By similarity). Guanine nucleotide exchange factor that activates SARA2. Required for the formation of COPII transport vesicles from the ER.
  • 组织特异性Ubiquitous.
  • 序列相似性Contains 3 WD repeats.
  • 细胞定位Nucleus. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Mammalian guanine nucleotide exchange factor mSec12 antibody
    • Preb antibody
    • PREB_HUMAN antibody
    • Prolactin regulatory binding element protein antibody
    • Prolactin regulatory element binding antibody
    • Prolactin regulatory element binding protein antibody
    • Prolactin regulatory element-binding protein antibody
    • SEC12 antibody
    see all

Anti-PREB antibody 图像



  • Predicted band size : 45 kDa

    Western blot detection of recombinant mouse PREB using ab3422.

  • ab3422 staining PREB in mouse skeletal tissue sections by Immunohistochemistry (Frozen sections)

    Tissue was fixed with acetone, blocked with 10% serum for 1 hour at 22°C and permeabilized with 1% Triton X-100. Samples were incubated with primary antibody (1/50 in 10% serum) for 12 hours at 4°C. A Rhodamine red-x conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

Anti-PREB antibody (ab3422)参考文献

ab3422 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Skeletal Muscle)
Specification Skeletal Muscle
Fixative Acetone
Permeabilization Yes - 1% Triton-X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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提交于 Jun 28 2012

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