概述

  • 产品名称Anti-Prealbumin抗体
    参阅全部 Prealbumin 一抗
  • 描述
    羊多克隆抗体to Prealbumin
  • 经测试应用适用于: IHC-P, Other, RID, Immunoelectrophoresis, Double Immunodiffusion, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig
  • 免疫原

    Human prealbumin purified from plasma.

  • 阳性对照
    • This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Glycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Antiserum is prepared by immunisation of sheep with Human Prealbumin and, if necessary, adsorbed to monospecificity by use of solid-phase adsorbents. An immunoglobulin fraction is then produced. The titre is adjusted so that inter-batch variation is within 10%. The product is 0.2µm filtered.
  • 克隆多克隆
  • 同种型IgG
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
RID Use at an assay dependent concentration.
Immunoelectrophoresis Use at an assay dependent concentration.
Double Immunodiffusion Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.

ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  • 组织特异性Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
  • 疾病相关Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
    Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
    Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
  • 序列相似性Belongs to the transthyretin family.
  • 结构域Each monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
  • 细胞定位Secreted. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amyloid polyneuropathy antibody
    • Amyloidosis I antibody
    • ATTR antibody
    • Carpal tunnel syndrome 1 antibody
    • CTS antibody
    • CTS1 antibody
    • Dysprealbuminemic euthyroidal hyperthyroxinemia antibody
    • Dystransthyretinemic hyperthyroxinemia antibody
    • Epididymis luminal protein 111 antibody
    • HEL111 antibody
    • HsT2651 antibody
    • PALB antibody
    • Prealbumin amyloidosis type I antibody
    • Prealbumin antibody
    • Prealbumin Thyroxine-binding antibody
    • Senile systemic amyloidosis antibody
    • TBPA antibody
    • Thyroxine binding prealbumin antibody
    • Transthyretin antibody
    • TTHY_HUMAN antibody
    • TTR antibody
    • TTR protein antibody
    see all

Anti-Prealbumin antibody 图像

  • ICC/IF image of ab9015 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9015 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- sheep (ab96939) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Prealbumin antibody (ab9015)参考文献

This product has been referenced in:
  • Teixeira CA  et al. Impairment of autophagy by TTR V30M aggregates: in vivo reversal by TUDCA and curcumin. Clin Sci (Lond) 130:1665-75 (2016). Read more (PubMed: 27382986) »
  • Hibert P  et al. Modifications in rat plasma proteome after remote ischemic preconditioning (RIPC) stimulus: identification by a SELDI-TOF-MS approach. PLoS One 9:e85669 (2014). Read more (PubMed: 24454915) »

See all 9 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Antigen retrieval step None
Sample Human Tissue sections (cartilage)
Specification cartilage
Permeabilization No
Fixative zinc buffered formalin
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Verified customer

提交于 Aug 20 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Antigen retrieval step None
Sample Mouse Tissue sections (Meniscus)
Specification Meniscus
Permeabilization No
Fixative zinc buffered formalin
Username

Abcam user community

Verified customer

提交于 Aug 08 2014

Thank you for your new contact.

It has not been specifically tested on frozen sections. However, since it is suitable for formalin-fixed paraffin embedded tissues, it is very likely that it works on frozen sections as well.

If you n...

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Thank you for your enquiry and your interest in our products.

I can confirm that ab9015 has been tested and characterized in IHC-P, RID, Immunoelectrophoresis, Double Immunodiffusion, WB and it recognizes pig (porcine) Prealbumin.

I...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human blood serum)
Loading amount 78 µg
Specification human blood serum
Gel Running Conditions Reduced Denaturing (15%, 6V, 45 min.)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Verified customer

提交于 Mar 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (serum)
Specification serum
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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Verified customer

提交于 Nov 30 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - other (serum)
Loading amount 40 µg
Specification serum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Apr 11 2006

The antibody has not been tested in western blotting yet. According to SwissProt the predicted MW of prealbumin is around 15kDa. I hope this information helps,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"