概述

  • 产品名称Anti-Pre4抗体
  • 描述
    小鼠多克隆抗体to Pre4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    Fusion protein:

    GVTYSSPTLATGFGAHMANPLLRKVVDRESDIPKTTVQVAEEAIVNAMRV LYYRDARSSRNFSLAIIDKNTGLTFKKNLQVENMKWDFAKDIKGYGTGKI

    , corresponding to amino acids 166-266 of Saccharomyces cerevisiae Pre4

  • 常规说明Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Constituents: 50% Glycerol
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 29 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 相关性The yeast 20S proteasome consists of 7 alpha and 7 beta subunits forming a highly ordered ring structure. Pre4 is one of the beta subunits (also known as beta7). The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Pre3 and Pre4 are necessary for post-acidic peptidase activity and Pre4 is also thought to play a role in mediating generation of the 20S proteasome from its precursors.
  • 细胞定位Cytoplasmic and Nuclear
  • 数据库链接
    • 别名
      • Macropain subunit PRE4 antibody
      • Multicatalytic endopeptidase complex subunit PRE4 antibody
      • Proteasome component PRE4 antibody
      • Proteinase YSCE subunit PRE4 antibody
      • YFR050C antibody
      see all

    Anti-Pre4 antibody (ab21639)参考文献

    ab21639 has not yet been referenced specifically in any publications.

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