概述

  • 产品名称
    Anti-PRDM5抗体
  • 描述
    兔多克隆抗体to PRDM5
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a portion of amino acids 100-200 of human PRDM5 protein

  • 阳性对照
    • Human colon tissue.

性能

应用

Our Abpromise guarantee covers the use of ab79016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 73 kDa.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能
    Sequence-specific DNA-binding transcription factor. Represses transcription at least in part by recruitment of the histone methyltransferase EHMT2/G9A and histone deacetylases such as HDAC1. Regulates hematopoiesis-associated protein-coding and microRNA (miRNA) genes.
  • 序列相似性
    Contains 16 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BCS2 antibody
    • PFM 2 antibody
    • PFM2 antibody
    • PR domain containing 5 antibody
    • PR domain containing protein 5 antibody
    • PR domain zinc finger protein 5 antibody
    • PR domain-containing protein 5 antibody
    • PRDM 5 antibody
    • PRDM5 antibody
    • PRDM5 protein antibody
    • PRDM5_HUMAN antibody
    see all

图片

  • ab79016, at 5 µg/ml, staining PRDM5 in formalin-fixed, paraffin-embedded human colon tissue by Immunohistochemistry.

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Human Cell (Human corneal endothelial cells (HCEC-12))
Specification
Human corneal endothelial cells (HCEC-12)
Permeabilization
No
Fixative
Paraformaldehyde
Username

Mr. Kostadin Rolev

Verified customer

提交于 Apr 29 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Peroxide block (3-4% Hydrogen peroxide) as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA based buffer - Epitope Retreival solution 2 (pH 9) for 20 min Cat No:AR9640
Sample
Human Tissue sections (Human corneas)
Specification
Human corneas
Permeabilization
Yes - Heat mediated at 100oC
Fixative
Formaldehyde
Username

Mr. Kostadin Rolev

Verified customer

提交于 Feb 07 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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