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Synthetic peptide: KVKQETVEPMDP, corresponding to C terminal amino acids 778-789 of Human PRDM1/Blimp1.
Our Abpromise guarantee covers the use of ab13700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|ChIP||Use at an assay dependent concentration. PubMed: 17264218|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).|
Ab13700 staining (1µg/ml) of human Lymph Node lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Human RPMI8226 cells stained with ab13700 at 5 µg/ml, incubated for 30 minutes at 4°C. In parallel, cells were stained with goat IgG at an equivalent concentration to that of PRDM1. The secondary antibody was a FITC-conjugated rabbit anti-goat IgG F(ab)2. PRDMI expression was analysed by flow cytometry.