Anti-PRDM1/Blimp1抗体(ab13700)
Key features and details
- Goat polyclonal to PRDM1/Blimp1
- Suitable for: ICC, ChIP, Flow Cyt
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PRDM1/Blimp1抗体
参阅全部 PRDM1/Blimp1 一抗 -
描述
山羊多克隆抗体to PRDM1/Blimp1 -
宿主
Goat -
经测试应用
适用于: ICC, ChIP, Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog, Pig -
免疫原
Synthetic peptide corresponding to Human PRDM1/Blimp1 aa 800 to the C-terminus (C terminal).
Database link: O75626 -
阳性对照
- ICC: A431 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13700于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use a concentration of 10 µg/ml.
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ChIP |
Use at an assay dependent concentration. PubMed: 17264218
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Flow Cyt | (1) |
Use at an assay dependent concentration.
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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ICC
Use a concentration of 10 µg/ml. |
ChIP
Use at an assay dependent concentration. PubMed: 17264218 |
Flow Cyt
Use at an assay dependent concentration. ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569). -
序列相似性
Belongs to the class V-like SAM-binding methyltransferase superfamily.
Contains 4 C2H2-type zinc fingers.
Contains 1 SET domain. -
翻译后修饰
Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 538384 Cow
- Entrez Gene: 481947 Dog
- Entrez Gene: 639 Human
- Entrez Gene: 12142 Mouse
- Entrez Gene: 100154284 Pig
- Entrez Gene: 309871 Rat
- Omim: 603423 Human
- SwissProt: O75626 Human
see all -
别名
- B Lymphocyte Induced Maturation Protein 1 antibody
- Beta interferon gene positive regulatory domain I binding factor antibody
- Beta-interferon gene positive regulatory domain I-binding factor antibody
see all
图片
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Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab13700 被引用在 10 文献中.
- Qing K et al. Dysregulated MDR1 by PRDM1/Blimp1 Is Involved in the Doxorubicin Resistance of Non-Germinal Center B-Cell-Like Diffuse Large B-Cell Lymphoma. Chemotherapy 67:12-23 (2022). PubMed: 34844236
- Li Q et al. PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells. Nat Commun 13:7677 (2022). PubMed: 36509766
- Okuzaki Y et al. PRDM14 and BLIMP1 control the development of chicken primordial germ cells. Dev Biol 455:32-41 (2019). PubMed: 31271752
- Chinnapaiyan S et al. TGF-ß1 increases viral burden and promotes HIV-1 latency in primary differentiated human bronchial epithelial cells. Sci Rep 9:12552 (2019). PubMed: 31467373
- Prajapati RS et al. PRDM1 controls the sequential activation of neural, neural crest and sensory progenitor determinants. Development 146:N/A (2019). PubMed: 31806661
- Zhao H et al. GPR39 marks specific cells within the sebaceous gland and contributes to skin wound healing. Sci Rep 5:7913 (2015). IF . PubMed: 25604641
- Beermann ML et al. Prdm1 (Blimp-1) and the expression of fast and slow myosin heavy chain isoforms during avian myogenesis in vitro. PLoS One 5:e9951 (2010). PubMed: 20376350
- Wang X et al. RelB NF-kappaB represses estrogen receptor alpha expression via induction of the zinc finger protein Blimp1. Mol Cell Biol 29:3832-44 (2009). PubMed: 19433448
- Yan J et al. BLIMP1 regulates cell growth through repression of p53 transcription. Proc Natl Acad Sci U S A 104:1841-6 (2007). ChIP ; Human . PubMed: 17264218
- Parekh S et al. BCL6 programs lymphoma cells for survival and differentiation through distinct biochemical mechanisms. Blood 110:2067-74 (2007). PubMed: 17545502