Anti-PRDM1/Blimp1抗体- ChIP Grade (ab106766)

概述

  • 产品名称Anti-PRDM1/Blimp1抗体- ChIP Grade
    参阅全部 PRDM1/Blimp1 一抗
  • 描述
    山羊多克隆抗体to PRDM1/Blimp1 - ChIP Grade
  • 经测试应用适用于: WB, ChIP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-DISDNADRLEDVED

    , corresponding to internal sequence amino acids 734-747 of Human PRDM1/Blimp1 (NP_001189.2)

  • 阳性对照
    • A431 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab106766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).
ChIP Use at an assay dependent concentration. PubMed: 23298838
IHC-P Use a concentration of 3 - 5 µg/ml.

靶标

  • 功能Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).
  • 序列相似性Belongs to the class V-like SAM-binding methyltransferase superfamily.
    Contains 4 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • 翻译后修饰Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.
    Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome.
  • 细胞定位Nucleus. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B Lymphocyte Induced Maturation Protein 1 antibody
    • Beta interferon gene positive regulatory domain I binding factor antibody
    • Beta-interferon gene positive regulatory domain I-binding factor antibody
    • BLIMP-1 antibody
    • BLIMP1 antibody
    • Positive Regulatory Domain I Binding Factor 1 antibody
    • Positive regulatory domain I-binding factor 1 antibody
    • PR Domain Containing 1 antibody
    • PR domain containing 1 with ZNF domain antibody
    • PR domain containing 1 with ZNF domain isoform 2 antibody
    • PR domain containing protein 1 antibody
    • PR domain zinc finger protein 1 antibody
    • PR domain-containing protein 1 antibody
    • PRDI BF1 antibody
    • PRDI binding factor 1 antibody
    • PRDI-BF1 antibody
    • PRDI-binding factor 1 antibody
    • PRDM 1 antibody
    • Prdm1 antibody
    • PRDM1_HUMAN antibody
    see all

Anti-PRDM1/Blimp1 antibody - ChIP Grade 图像

  • Anti-PRDM1/Blimp1 antibody - ChIP Grade (ab106766) at 0.3 µg/ml + A431 lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 92 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining PRDM1/Blimp1 with ab106766 at 3.8 µg/ml.

Anti-PRDM1/Blimp1 antibody - ChIP Grade (ab106766)参考文献

This product has been referenced in:
  • Kim SJ  et al. Regulation of dendritic cell activation by microRNA let-7c and BLIMP1. J Clin Invest N/A:N/A (2013). ChIP ; Mouse . Read more (PubMed: 23298838) »

See 1 Publication for this product

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