The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).
Use at an assay dependent concentration. PubMed: 23298838
Use a concentration of 3 - 5 µg/ml.
功能Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569).
序列相似性Belongs to the class V-like SAM-binding methyltransferase superfamily. Contains 4 C2H2-type zinc fingers. Contains 1 SET domain.
翻译后修饰Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation. Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome.