The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml.
Use a concentration of 2.5 µg/ml.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 140 kDa.
Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. Isoform 4 may regulate granulocytes differentiation.
Expressed in uterus and kidney.
Note=A chromosomal aberration involving PRDM16 is found in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Reciprocal translocation t(1;3)(p36;q21). Isoform 4 is specifically expressed in adult T-cell leukemia.
Contains 10 C2H2-type zinc fingers. Contains 1 SET domain.
ab106410 at 2.5 µg/ml, staining PRDM16 in paraffin embedded rat brain tissue.
Immunocytochemistry/ Immunofluorescence - Anti-PRDM16 antibody (ab106410)Image is courtesy of an Abreview submitted by Lena Appelsved
ab106410 staining RAP1 in Human Brown adipocytes from supraclavicular fat tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum, 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (2 ug/ml in 10% serum, 1% BSA, 0.3% Triton X100, PBS) for 16 hours at 4°C. A Cy3®-conjugated Donkey F(ab')2 anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Monnier C et al. The nuclear retinoid-related orphan receptor RORa controls circadian thermogenic programming in white fat depots. Physiol Rep6:e13678 (2018).
Read more (PubMed: 29673115) »
Cao W et al. Homeobox a5 Promotes White Adipose Tissue Browning Through Inhibition of the Tenascin C/Toll-Like Receptor 4/Nuclear Factor Kappa B Inflammatory Signaling in Mice. Front Immunol9:647 (2018).
Read more (PubMed: 29651293) »