概述

  • 产品名称Anti-PRDM16抗体
    参阅全部 PRDM16 一抗
  • 描述
    兔多克隆抗体to PRDM16
  • 特异性At least four isoforms of PRDM16 are known to exist; ab106410 will recognize all four.
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 17 amino acid peptide from near the carboxy terminus of Human PRDM16 (CAH71530).

  • 阳性对照
    • Rat brain tissue

性能

应用

Our Abpromise guarantee covers the use of ab106410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 140 kDa.

靶标

  • 功能Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. Isoform 4 may regulate granulocytes differentiation.
  • 组织特异性Expressed in uterus and kidney.
  • 疾病相关Note=A chromosomal aberration involving PRDM16 is found in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Reciprocal translocation t(1;3)(p36;q21). Isoform 4 is specifically expressed in adult T-cell leukemia.
  • 序列相似性Contains 10 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CMD1LL antibody
    • KIAA1675 antibody
    • LVNC8 antibody
    • MDS1/EVI1 like gene 1 antibody
    • MDS1/EVI1-like gene 1 antibody
    • MEL1 antibody
    • PFM 13 antibody
    • PFM13 antibody
    • PR domain containing 16 antibody
    • PR domain containing protein 16 antibody
    • PR domain zinc finger protein 16 antibody
    • PR domain-containing protein 16 antibody
    • PRD16_HUMAN antibody
    • Prdm16 antibody
    • Transcription factor MEL 1 antibody
    • Transcription factor MEL1 antibody
    see all

Anti-PRDM16 antibody 图像

  • Lane 1 : Anti-PRDM16 antibody (ab106410) at 1 µg/ml
    Lane 2 : Anti-PRDM16 antibody (ab106410) at 2 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 140 kDa
  • ab106408 at 2.5 µg/ml, staining PRDM16 in paraffin embedded Rat brain tissue.
  • ab106410 staining RAP1 in Human Brown adipocytes from supraclavicular fat tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum, 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (2 ug/ml in 10% serum, 1% BSA, 0.3% Triton X100, PBS) for 16 hours at 4°C. A Cy3®-conjugated Donkey F(ab')2 anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-PRDM16 antibody (ab106410) at 1/2500 dilution (12 hours at 4°C, 1% skim milk) + Mouse MEL whole cell lysate at 17 µg with Milk, 1 hour at 25°C at 5 %

    Secondary
    HRP anti-rabbit IgG
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 140 kDa
    Additional bands at : 140 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    Image courtesy of an anonymous Abreview

    See Abreview

  • Immunofluorescence of PRDM16 in Human Brain cells using ab106410 at 20 ug/ml.

Anti-PRDM16 antibody (ab106410)参考文献

This product has been referenced in:
  • Suárez J  et al. Oleoylethanolamide enhances ß-adrenergic-mediated thermogenesis and white-to-brown adipocyte phenotype in epididymal white adipose tissue in rat. Dis Model Mech 7:129-41 (2014). WB ; Rat . Read more (PubMed: 24159189) »
  • Hondares E  et al. Peroxisome Proliferator-activated Receptor a (PPARa) Induces PPAR? Coactivator 1a (PGC-1a) Gene Expression and Contributes to Thermogenic Activation of Brown Fat: INVOLVEMENT OF PRDM16. J Biol Chem 286:43112-22 (2011). WB ; Mouse . Read more (PubMed: 22033933) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 17 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (MEL)
Specification MEL
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 29 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (endothelial cell)
Specification endothelial cell
Permeabilization Yes - tween-20
Fixative Paraformaldehyde
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提交于 Oct 29 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step 10% serum,1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Human Cell (Brown adipocytes from supraclavicular fat tissue)
Specification Brown adipocytes from supraclavicular fat tissue
Permeabilization Yes - 0.3% Triton X100
Fixative Paraformaldehyde
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Mrs. Lena Appelsved

Verified customer

提交于 Nov 21 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human ductal carcinoma)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Permeabilization Yes - tween-20
Specification Human ductal carcinoma
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

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提交于 May 11 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Sample Mouse Tissue sections (Lymph node)
Specification Lymph node
Permeabilization Yes - tween-20
Fixative Paraformaldehyde
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Abcam user community

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提交于 Oct 29 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (10.5%)
Sample Mouse Tissue lysate - other (Brown adipose tissue)
Specification Brown adipose tissue
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jul 12 2013

Thank you for contacting us.

I have contacted the originating lab on your behalf. While the exact sequence is proprietary, but the immunogen is from aa1101-1160 of Human PRDM16 (CAH71530).

I hope this information is helpful to you. P...

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Thank you for your reply.
I am very pleased that I could help you with your case.
Regarding your WB and the provided information, I am pretty sure you are getting the expected results.
Please feel free to contact us again when ever you n...

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Thank you for contacting us.
There are four isoforms of the human protein, but they all have the same C-terminus sequence from which the immunogen for this antibody is derived. I compared the immunogen sequence with the mouse sequence and they hav...

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Thank you for your reply.

I can confirm that ab106410 has been raised against a peptide corresponding to human PRDM16. The antibody will cross-react with rat PRDM16, as shown in western blot and IHC on the datasheet. I do not have details of...

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