Anti-PRDM14抗体(ab91587)

概述

  • 产品名称Anti-PRDM14抗体
    参阅全部 PRDM14 一抗
  • 描述
    兔多克隆抗体to PRDM14
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human PRDM14.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat; Raji; Ramos; HuES7; A549; HeLa; HepG2.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab91587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).

靶标

  • 功能May be involved in transcriptional regulation.
  • 序列相似性Contains 6 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC59730 antibody
    • PFM 11 antibody
    • PFM11 antibody
    • PR domain containing 14 antibody
    • PR domain zinc finger protein 14 antibody
    • PR domain-containing protein 14 antibody
    • PRD14_HUMAN antibody
    • PRDM 14 antibody
    • PRDM14 antibody
    see all

Anti-PRDM14 antibody 图像

  • All lanes : Anti-PRDM14 antibody (ab91587) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 4 : HuES7 Whole Cell Lysate
    Lane 5 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 7 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab91587 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91587, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 cells at 5µg/ml.

Anti-PRDM14 antibody (ab91587)参考文献

ab91587 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (8)
Sample Human Cell lysate - whole cell (Couple of different cell lines - HS478, MCF7, MDAM)
Specification Couple of different cell lines - HS478, MCF7, MDAM
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Dec 23 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Wako target retrieval solution
Sample Marmoset (common) Tissue sections (adult testis)
Specification adult testis
Permeabilization No
Fixative Formaldehyde
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煜欽 林

Verified customer

提交于 Dec 19 2013

I really hope that this anitbody will work for you. Please let me know if there is anything else I can do. The testing discount information is below: DISCOUNT CODE: ******** Expiration date: ****** I am very pleased to hear you...

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The discount is for the price of one primary antibody of your choosing. When you submit an Abreview, the discount code will become active and then you will be eligible for 1 antibody free of charge. Please feel free to contact me if you have any...

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Thank you for your reply. As ab113444 is untested it does not come under our Abpromise and so we cannot guarantee that you would get a refund if it does not work. What we can offer you though is our testing discount, if you are interested. ...

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Thank you for contacting Abcam about ab91587. Unfortunately the anti-PRDM14 antibody has not been tested on mouse tissue and I have done an alignment between the human and mouse PRDM14 sequences and they share ~80% homology. However between hum...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human ES cell)
Loading amount 50 µg
Specification human ES cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"