概述

  • 产品名称Anti-PRDM14抗体
    参阅全部 PRDM14 一抗
  • 描述
    兔多克隆抗体to PRDM14
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    A region within a synthetic peptide: ASLHHAISGL LVPPDSSGSD SLPQTLDKDS LQLPEGLCLM QTVFGEVPHF, corresponding to internal sequence amino acids 217-266 of Human PRDM14

  • 阳性对照
    • Jurkat cell lysate for Western blot, Human lung tissue for Immunohistochemistry (paraffin embedded).

性能

应用

Our Abpromise guarantee covers the use of ab28638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

Anti-PRDM14 antibody 图像

  • Anti-PRDM14 antibody (ab28638) at 1.25 µg/ml + Jurkat cell lysate 10ug.

    Secondary
    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size : 64 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.
  • ab28638 staining PRDM14 in Human lung by Immunohistochemistry (FFPE-sections) at 4-8ug/ml. Positive label Alveolar cells (arrows).

Anti-PRDM14 antibody (ab28638)参考文献

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (293T-FLAG-hPRDM14)
Loading amount 15 µg
Specification 293T-FLAG-hPRDM14
Treatment transcient transfected 293T with FLAG-hPRDM14
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 19 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"