Mouse, Rat, Rabbit, Cow, Cat, Dog, Pig
A region within synthetic peptide: VKSRHPGQSL LAKAGDGPGA EPGYPPEPGD PKSDDSDVDV CFTDDQSDPE, corresponding to C terminal amino acids 649-698 of Human PRDM13
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1.25 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 75 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
PRDM13 may be involved in transcriptional regulation.
- MU MB 20.220 antibody
- PFM 10 antibody
- PFM10 antibody
Western blot - PRDM13 antibody (ab66665)
All lanes :
Anti-PRDM13 antibody (ab66665) at 1.25 µg/mlLane 1 :
MarkerLane 2 :
Jurkat cell lysate at 10 µgSecondaryAll lanes :
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
75 kDaObserved band size:
68 kDa (why is the actual band size different from the predicted?
)Additional bands at:
20 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
has not yet been referenced specifically in any publications.
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