Anti-PRDM13抗体(ab66665)

概述

  • 产品名称Anti-PRDM13抗体
    参阅全部 PRDM13 一抗
  • 描述
    兔多克隆抗体to PRDM13
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: VKSRHPGQSL LAKAGDGPGA EPGYPPEPGD PKSDDSDVDV CFTDDQSDPE, corresponding to C terminal amino acids 649-698 of Human PRDM13

  • 阳性对照
    • Jurkat cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab66665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 75 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

Anti-PRDM13 antibody 图像

  • All lanes : Anti-PRDM13 antibody (ab66665) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 75 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,36 kDa. We are unsure as to the identity of these extra bands.

Anti-PRDM13 antibody (ab66665)参考文献

ab66665 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Sample Mouse Cell (Two Cell Embryo)
Specification Two Cell Embryo
Permeabilization Yes - 0.5% Triton for 10 min
Fixative Paraformaldehyde
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Dr. jesse mager

Verified customer

提交于 Jan 08 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"