Anti-PRDM13抗体(ab66665)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to PRDM13
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: VKSRHPGQSL LAKAGDGPGA EPGYPPEPGD PKSDDSDVDV CFTDDQSDPE, corresponding to C terminal amino acids 649-698 of Human PRDM13

  • 阳性对照
    • Jurkat cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 75 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

Anti-PRDM13 antibody 图像

  • All lanes : Anti-PRDM13 antibody (ab66665) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 75 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,36 kDa. We are unsure as to the identity of these extra bands.

实验方案

Anti-PRDM13 antibody (ab66665)参考文献

ab66665 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Sample
Mouse Cell (Two Cell Embryo)
Specification
Two Cell Embryo
Permeabilization
Yes - 0.5% Triton for 10 min
Fixative
Paraformaldehyde
Username

Dr. Jesse Mager

Verified customer

提交于 Jan 08 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册