Anti-PRDM10抗体[48AT1224.90.46] (ab3787)

概述

  • 产品名称Anti-PRDM10抗体[48AT1224.90.46]
    参阅全部 PRDM10 一抗
  • 描述
    小鼠单克隆抗体[48AT1224.90.46] to PRDM10
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human PRDM10 aa 50-79 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • 293 cell lysate.
  • 常规说明


    Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液PBS with 0.1% sodium azide
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明This antibody is purified through a protein G column and eluted out with both high and low pH buffers. It is neutralized immediately after elution, followed by dialysis against PBS.
  • Primary antibody说明Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.
  • 克隆单克隆
  • 克隆编号48AT1224.90.46
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 120.7 kDa.
ELISA 1/1000.

靶标

  • 功能May be involved in transcriptional regulation.
  • 序列相似性Contains 10 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • 结构域The SET domain is degenerated, suggesting that it has lost mehtyltransferase activity.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA1231 antibody
    • MGC131802 antibody
    • PFM7 antibody
    • PR domain containing 10 antibody
    • PR domain containing protein 10 antibody
    • PR domain family member 7 antibody
    • PR domain zinc finger protein 10 antibody
    • PR domain-containing protein 10 antibody
    • PRD10_HUMAN antibody
    • PRDM zinc finger transcription factor antibody
    • prdm10 antibody
    • TRIS antibody
    • Tristanin antibody
    see all

Anti-PRDM10 antibody [48AT1224.90.46] 图像

  • Anti-PRDM10 antibody [48AT1224.90.46] (ab3787) + Extracts from 293 cells

    Predicted band size : 120.7 kDa

Anti-PRDM10 antibody [48AT1224.90.46] (ab3787)参考文献

ab3787 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116 cells)
Loading amount 50 µg
Specification HCT116 cells
Gel Running Conditions Reduced Denaturing (6-15% gradient gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Sep 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"