概述

  • 产品名称Anti-PRCP抗体
    参阅全部 PRCP 一抗
  • 描述
    小鼠多克隆抗体to PRCP
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human PRCP protein (NP_005031)

  • 阳性对照
    • Human kidney tissue lysate and transfected 293T cell line lysate

性能

应用

Our Abpromise guarantee covers the use of ab72942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Detects a band of approximately 37 kDa (tissue lysate) and 57 kDa (predicted molecular weight: 57 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
    • 组织特异性Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney.
    • 序列相似性Belongs to the peptidase S28 family.
    • 细胞定位Lysosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Angiotensinase C antibody
      • HUMPCP antibody
      • Lysosomal carboxypeptidase C antibody
      • Lysosomal Pro X carboxypeptidase antibody
      • Lysosomal Pro-X carboxypeptidase antibody
      • MGC2202 antibody
      • PCP antibody
      • PCP_HUMAN antibody
      • PRCP antibody
      • Proline carboxypeptidase antibody
      • Prolylcarboxypeptidase (angiotensinase C) antibody
      • Prolylcarboxypeptidase antibody
      • prolylcarboxypeptidase isoform 1 preproprotein antibody
      see all

    Anti-PRCP antibody 图像

    • Anti-PRCP antibody (ab72942) at 1/500 dilution + human kidney tissue lysate at 25 µg

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 57 kDa
      Observed band size : 57 kDa
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
    • All lanes : Anti-PRCP antibody (ab72942) at 1/500 dilution

      Lane 1 : Transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

      Predicted band size : 57 kDa
      Observed band size : 57 kDa

    Anti-PRCP antibody (ab72942)参考文献

    This product has been referenced in:
    • Duan L  et al. Prolylcarboxypeptidase Regulates Proliferation, Autophagy, and Resistance to 4-Hydroxytamoxifen-induced Cytotoxicity in Estrogen Receptor-positive Breast Cancer Cells. J Biol Chem 286:2864-76 (2011). WB ; Human . Read more (PubMed: 21087932) »

    See 1 Publication for this product

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