Anti-PRC1 (phospho T481)抗体[EP1514Y] (ab62366)


  • 产品名称Anti-PRC1 (phospho T481)抗体[EP1514Y]
    参阅全部 PRC1 一抗
  • 描述
    兔单克隆抗体[EP1514Y] to PRC1 (phospho T481)
  • 特异性ab62366 detects PRC1 phosphorylated at Theonine 481.
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A phosphopeptide corresponding to residues surrounding Threonine 481 of human PRC1

  • 阳性对照
    • HeLa cells and HeLa cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EP1514Y
  • 同种型IgG
  • 研究领域



Our Abpromise guarantee covers the use of ab62366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/500. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
    • 序列相似性Belongs to the MAP65/ASE1 family.
    • 结构域Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.
    • 翻译后修饰Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.
    • 细胞定位Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Anaphase spindle elongation 1 homolog antibody
      • ASE1 antibody
      • PRC1 antibody
      • PRC1_HUMAN antibody
      • Protein regulating cytokinesis 1 antibody
      • Protein regulator of cytokinesis 1 antibody
      see all

    Anti-PRC1 (phospho T481) antibody [EP1514Y] 图像

    • Primary ab dilution 1:1000 dilution. Secondary ab description and code (ab id) Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051). Secondary ab dilution 1:100,000 dilution. Blocking buffer and concentration 5% NFDM/TBST. Lane 1: PRC1 (pT481) phospho peptide Lane 2: PRC1 non-phospho peptide. Exposure time 3 minutes

    • All lanes : Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366) at 1/500 dilution

      Lane 1 : HeLa cell lysates (untreated)
      Lane 2 : HeLa cell lysates treated with aphidicolin + nocodazole

      Lysates/proteins at 10 µg per lane.

      goat anti-rabbit HRP labeled, at 1/2000 dilution

      Predicted band size : 72 kDa
      Observed band size : 72 kDa

    Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366)参考文献

    This product has been referenced in:
    • Cundell MJ  et al. A PP2A-B55 recognition signal controls substrate dephosphorylation kinetics during mitotic exit. J Cell Biol 214:539-54 (2016). Read more (PubMed: 27551054) »

    See 1 Publication for this product

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