Anti-PRC1 (phospho T481)抗体[EP1514Y] (ab62366)

概述

• 产品名称
  Anti-PRC1 (phospho T481)抗体[EP1514Y]
  参阅全部 PRC1 一抗
• 描述
  兔单克隆抗体[EP1514Y] to PRC1 (phospho T481)
• 特异性
  ab62366 detects PRC1 phosphorylated at Theonine 481.
• 经测试应用
  适用于: ICC/IF, WB, IHC-Pmore details
  不适用于: Flow Cyt or IP
• 种属反应性
  与反应: Human
  
• 免疫原

  A phosphopeptide corresponding to residues surrounding Threonine 481 of human PRC1

• 阳性对照
  • HeLa cells and HeLa cell lysates.
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

   

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
• 存储溶液
  PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• 纯度
  Tissue culture supernatant
• 克隆
  单克隆
• 克隆编号
  EP1514Y
• 同种型
  IgG
• 研究领域
  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Cytokinesis

相关产品

• Positive Controls
  • HeLa nuclear extract lysate (ab14655)

应用

靶标

• 功能
  Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
• 序列相似性
  Belongs to the MAP65/ASE1 family.
• 结构域
  Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.
• 翻译后修饰
  Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.
• 细胞定位
  Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.

• Target information above from: UniProt accession O43663 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 9055 Human
  • Omim: 603484 Human
  • SwissProt: O43663 Human
  • Unigene: 366401 Human
• 别名
  • Anaphase spindle elongation 1 homolog antibody
  • ASE1 antibody
  • PRC1 antibody

  • PRC1_HUMAN antibody
  • Protein regulating cytokinesis 1 antibody
  • Protein regulator of cytokinesis 1 antibody

  see all

实验方案

• Immunohistochemistry protocols
• Immunocytochemistry & immunofluorescence protocols
• Western blot protocols