Anti-PRC1 (phospho T481)抗体[EP1514Y] (ab62366)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-PRC1 (phospho T481)抗体[EP1514Y]
参阅全部 PRC1 一抗 -
描述兔单克隆抗体[EP1514Y] to PRC1 (phospho T481)
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宿主Rabbit
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特异性ab62366 detects PRC1 phosphorylated at Theonine 481.
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经测试应用适用于: ICC/IF, WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性与反应: Human
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免疫原
A phosphopeptide corresponding to residues surrounding Threonine 481 of human PRC1
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阳性对照
- HeLa cells and HeLa cell lysates.
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常规说明
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EP1514Y
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同种型IgG
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研究领域
相关产品
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab62366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/500. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
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序列相似性Belongs to the MAP65/ASE1 family.
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结构域Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.
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翻译后修饰Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.
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细胞定位Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
- Information by UniProt
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数据库链接
- Entrez Gene: 9055 Human
- Omim: 603484 Human
- SwissProt: O43663 Human
- Unigene: 366401 Human
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别名
- Anaphase spindle elongation 1 homolog antibody
- ASE1 antibody
- PRC1 antibody
see all
图片
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![Dot Blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366)](http://www.abcam.cn/ps/products/62/ab62366/Images/ab62366-280631-ab62366dot-002.jpg)
Dot Blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366)Primary ab dilution 1:1000 dilution. Secondary ab description and code (ab id) Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051). Secondary ab dilution 1:100,000 dilution. Blocking buffer and concentration 5% NFDM/TBST. Lane 1: PRC1 (pT481) phospho peptide Lane 2: PRC1 non-phospho peptide. Exposure time 3 minutes
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![Western blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366)](http://www.abcam.cn/ps/products/62/ab62366/Images/ab62366-75694-ab62366-WB-002.jpg)
Western blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366)All lanes : Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366) at 1/500 dilution
Lane 1 : HeLa cell lysates (untreated)
Lane 2 : HeLa cell lysates treated with aphidicolin + nocodazole
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP labeled, at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa
实验方案
文献
This product has been referenced in:
- Cundell MJ et al. A PP2A-B55 recognition signal controls substrate dephosphorylation kinetics during mitotic exit. J Cell Biol 214:539-54 (2016). Read more (PubMed: 27551054) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"