Anti-PRC1抗体[6G2] (ab39069)

概述

  • 产品名称
    Anti-PRC1抗体[6G2]
    参阅全部 PRC1 一抗
  • 描述
    小鼠单克隆抗体[6G2] to PRC1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 1/150 of Human PRC1

  • 阳性对照
    • HeLa cell extract

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    ab39069 is an affinity purified IgG.
  • 克隆
    单克隆
  • 克隆编号
    6G2
  • 同种型
    IgG2b
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab39069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 72 kDa.

靶标

  • 功能
    Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody.
  • 序列相似性
    Belongs to the MAP65/ASE1 family.
  • 结构域
    Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain.
  • 翻译后修饰
    Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization.
  • 细胞定位
    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Anaphase spindle elongation 1 homolog antibody
    • ASE1 antibody
    • PRC1 antibody
    • PRC1_HUMAN antibody
    • Protein regulating cytokinesis 1 antibody
    • Protein regulator of cytokinesis 1 antibody
    see all

Anti-PRC1 antibody [6G2] 图像

  • Anti-PRC1 antibody [6G2] (ab39069) at 1 µg/ml + Hela cell extracts

    Secondary
    goat anti-mouse secondary conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 72 kDa
    Observed band size : 66 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-PRC1 antibody [6G2] (ab39069)参考文献

ab39069 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Treatment
Benzonase on lysate
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jan 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册