Anti-PRC1抗体[6G2] (ab39069)
Key features and details
- Mouse monoclonal [6G2] to PRC1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-PRC1抗体[6G2]
参阅全部 PRC1 一抗 -
描述
小鼠单克隆抗体[6G2] to PRC1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide, corresponding to amino acids 1/150 of Human PRC1
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阳性对照
- HeLa cell extract
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
ab39069 is an affinity purified IgG. -
克隆
单克隆 -
克隆编号
6G2 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab39069于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 72 kDa.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 72 kDa. |
靶标
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功能
Cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. -
序列相似性
Belongs to the MAP65/ASE1 family. -
结构域
Microtubule binding occurs via a basic patch in the central spectrin-like domain and requires also the unstructured C-terminal domain. -
翻译后修饰
Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization. -
细胞定位
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis. - Information by UniProt
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数据库链接
- Entrez Gene: 9055 Human
- Omim: 603484 Human
- SwissProt: O43663 Human
- Unigene: 366401 Human
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别名
- Anaphase spindle elongation 1 homolog antibody
- ASE1 antibody
- PRC1 antibody
see all
图片
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Anti-PRC1 antibody [6G2] (ab39069) at 1 µg/ml + Hela cell extracts
Secondary
goat anti-mouse
secondary conjugated to HRP
Developed using the ECL technique.
Predicted band size: 72 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (0)
ab39069 尚未被引用在任何文献中。