概述

  • 产品名称Anti-PRAS40抗体
    参阅全部 PRAS40 一抗
  • 描述
    兔多克隆抗体to PRAS40
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 226-256 of Human PRAS40 (NP_001092103.1, NP_115751.2 NP_001092102.1).

  • 阳性对照
    • HeLa cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab123294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 27 kDa.

靶标

  • 功能Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
  • 组织特异性Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
  • 翻译后修饰Phosphorylated by AKT1. Phosphorylation relieves inhibitory function on mTORC1.
  • 细胞定位Cytoplasm > cytosol. Found in the cytosolic fraction of the brain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 40 kDa proline rich AKT substrate antibody
    • 40 kDa proline-rich AKT substrate antibody
    • AKT1 S1 antibody
    • AKT1 substrate 1 (proline rich) antibody
    • AKT1 substrate 1 antibody
    • AKT1S 1 antibody
    • AKT1S1 antibody
    • AKTS1_HUMAN antibody
    • Lobe antibody
    • MGC2865 antibody
    • PRAS 40 antibody
    • PRAS antibody
    • PRAS40 antibody
    • Proline rich akt substrate antibody
    • Proline rich Akt substrate 40 kDa antibody
    • Proline-rich AKT1 substrate 1 antibody
    see all

Anti-PRAS40 antibody 图像

  • Anti-PRAS40 antibody (ab123294) at 1/100 dilution + HeLa cell lysate at 35 µg

    Predicted band size : 27 kDa

Anti-PRAS40 antibody (ab123294)参考文献

ab123294 has not yet been referenced specifically in any publications.

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