概述
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产品名称Anti-PRAME抗体
参阅全部 PRAME 一抗 -
描述小鼠多克隆抗体to PRAME
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宿主Mouse
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经测试应用适用于: IHC-P, WBmore details
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种属反应性与反应: Human
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免疫原
Full length Human PRAME (NP_006106.1, amino acids 1-509).
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阳性对照
- HeLa cell lysate, PRAME transfected 293T cell lysate.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... -
纯度Protein A purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab89097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/40. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See Abreview | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. |
靶标
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功能Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.
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组织特异性Expressed in testis. Detected in samples of kidney, brain and skin.
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序列相似性Belongs to the PRAME family.
Contains 4 LRR (leucine-rich) repeats. -
细胞定位Nucleus. Cell membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 23532 Human
- Omim: 606021 Human
- SwissProt: P78395 Human
- Unigene: 30743 Human
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别名
- 4930534P07Rik antibody
- Cancer/testis antigen 130 antibody
- CT130 antibody
see all
图片
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Western blot - Anti-PRAME antibody (ab89097)Anti-PRAME antibody (ab89097) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 58 kDa
Observed band size: 65 kDa (why is the actual band size different from the predicted?) -
Western blot - Anti-PRAME antibody (ab89097)All lanes : Anti-PRAME antibody (ab89097) at 1 µg/ml
Lane 1 : PRAME transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 58 kDa
Observed band size: 60 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAME antibody (ab89097)This image is courtesy of an anonymous Abreviewab89097 staining PRAME in Human Melanoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a Tris/EDTA buffer. Samples were incubated with primary antibody (1/40) for 1 hour 30 minutes at 28°C.
文献
ab89097 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"