Anti-PRAME抗体(ab89097)
Key features and details
- Mouse polyclonal to PRAME
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PRAME抗体
参阅全部 PRAME 一抗 -
描述
小鼠多克隆抗体to PRAME -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human PRAME. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- HeLa whole cell lysate (ab150035), PRAME transfected 293T cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab89097于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. |
靶标
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功能
Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. -
组织特异性
Expressed in testis. Detected in samples of kidney, brain and skin. -
序列相似性
Belongs to the PRAME family.
Contains 4 LRR (leucine-rich) repeats. -
细胞定位
Nucleus. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 23532 Human
- Omim: 606021 Human
- SwissProt: P78395 Human
- Unigene: 30743 Human
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别名
- 4930534P07Rik antibody
- Cancer/testis antigen 130 antibody
- CT130 antibody
see all
图片
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Anti-PRAME antibody (ab89097) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 58 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
All lanes : Anti-PRAME antibody (ab89097) at 1 µg/ml
Lane 1 : PRAME transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (2)
ab89097 被引用在 2 文献中.
- Sivaccumar JP et al. Development of a New Highly Selective Monoclonal Antibody against Preferentially Expressed Antigen in Melanoma (PRAME) and Identification of the Target Epitope by Bio-Layer Interferometry. Int J Mol Sci 22:N/A (2021). PubMed: 33804612
- Nettersheim D et al. The cancer/testis-antigen PRAME supports the pluripotency network and represses somatic and germ cell differentiation programs in seminomas. Br J Cancer 115:454-64 (2016). PubMed: 27441500