概述

  • 产品名称Anti-PRAM1抗体
    参阅全部 PRAM1 一抗
  • 描述
    山羊多克隆抗体to PRAM1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    DFCDPLENQPLPLGR

    , corresponding to C terminal amino acids 656-670 of Human PRAM1.

  • 阳性对照
    • Jurkat cell lysates.
  • 常规说明Principal Names - PRAM-1; PML-RARA target gene encoding an Adaptor Molecule-1 Official Gene Symbol - PRAM-1 GenBank Accession Number – NP_115528 LocusLink ID - 84106


    PRAM-1 is an adaptor protein interacting with proteins involved in an ATRA-signaling pathway

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody说明PRAM-1 is an adaptor protein interacting with proteins involved in an ATRA-signaling pathway
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with Human PRAM1 peptide (ab22785).

Approx 90-100kDa band seen in Jurkat cell lysates. Overnight incubation is recommended with this antibody.

Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001; 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97kDa and this is what we observed as well.

靶标

  • 功能May be involved in myeloid differentiation. May be involved in integrin signaling in neutrophils. Binds to PtdIns(4)P.
  • 组织特异性Expressed in peripheral blood leukocytes and bone marrow. Expressed in monocytes, and to a lesser extent in granulocytes and lymphocytes. Not expressed in non hematopoietic tissues except in lung.
  • 序列相似性Contains 1 SH3 domain.
  • 结构域The SH3 domain binds to PtdIns(4)P.
  • 翻译后修饰May be phosphorylated on tyrosines.
  • Information by UniProt
  • 数据库链接
  • 别名
    • PML RAR antibody
    • PML RARA regulated adaptor molecule 1 antibody
    • PML RARA target gene encoding an Adaptor Molecule 1 antibody
    • PML-RARA-regulated adapter molecule 1 antibody
    • PRAM 1 antibody
    • PRAM antibody
    • PRAM-1 antibody
    • PRAM_HUMAN antibody
    • PRAM1 antibody
    see all

Anti-PRAM1 antibody 图像

  • staining (2µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence

Anti-PRAM1 antibody (ab805)参考文献

ab805 has not yet been referenced specifically in any publications.

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