产品名称 Anti-PRAM1抗体 参阅全部 PRAM1 一抗
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DFCDPLENQPLPLGR , corresponding to C terminal amino acids 656-670 of Human PRAM1.
常规说明 Principal Names - PRAM-1; PML-RARA target gene encoding an Adaptor Molecule-1
Official Gene Symbol - PRAM-1
GenBank Accession Number – NP_115528
LocusLink ID - 84106
PRAM-1 is an adaptor protein interacting with proteins involved in an ATRA-signaling pathway
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
Concentration information loading...
纯度 IgG fraction
纯化说明 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Primary antibody说明 PRAM-1 is an adaptor protein interacting with proteins involved in an ATRA-signaling pathway
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Can be blocked with
Human PRAM1 peptide (ab22785).
Approx 90-100kDa band seen in Jurkat cell lysates. Overnight incubation is recommended with this antibody.
Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001; 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97kDa and this is what we observed as well.
功能 May be involved in myeloid differentiation. May be involved in integrin signaling in neutrophils. Binds to PtdIns(4)P.
组织特异性 Expressed in peripheral blood leukocytes and bone marrow. Expressed in monocytes, and to a lesser extent in granulocytes and lymphocytes. Not expressed in non hematopoietic tissues except in lung.
序列相似性 Contains 1 SH3 domain.
结构域 The SH3 domain binds to PtdIns(4)P.
翻译后修饰 May be phosphorylated on tyrosines.
Information by UniProt
PML RAR antibody
PML RARA regulated adaptor molecule 1 antibody
PML RARA target gene encoding an Adaptor Molecule 1 antibody
Anti-PRAM1 antibody 图像
Western blot - Anti-PRAM1 antibody (ab805)
µg/ml) of Jurkat lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence
Anti-PRAM1 antibody (ab805)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"