兔多克隆抗体to PRAK (phospho S212)
Synthetic phosphopeptide corresponding to amino acid residues surrounding S212 region of human PRAK conjugated to KLH
Human hepatocarcinoma tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Protein G purified
ab59999 is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2 step procedure with the control and phosphorylated peptides. The phospho specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
Mediates stress-induced small heat shock protein 27 phosphorylation.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
Cytoplasm. Nucleus. Also observed in the nucleus.
Information by UniProt
MAP kinase-activated protein kinase 5 antibody
MAPK-activated protein kinase 5 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRAK (phospho S212) antibody (ab59999)
ab59999 at 1/50 dilution staining PRAK in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
The primary antibody was peroxidase conjugated to the secondary antibody, followed by AEC staining.
has not yet been referenced specifically in any publications.
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