Mouse, Rat, Pig
Recombinant fragment corresponding to a region within amino acids 197-467 of Human PRAK (AAH47284).
- WB: 293T and H1299 whole cell lysates; A431, HeLaS3, HepG2, MOLT4 and Raji cell lines
ICC/IF: HeLa cells
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: 54 kDa.
||1/100 - 1/200.
Mediates stress-induced small heat shock protein 27 phosphorylation.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
Cytoplasm. Nucleus. Also observed in the nucleus.
Information by UniProt
- MAP kinase-activated protein kinase 5 antibody
- MAPK-activated protein kinase 5 antibody
- MAPK5_HUMAN antibody
Western blot - Anti-PRAK antibody (ab97332)
All lanes : Anti-PRAK antibody (ab97332) at 1/500 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PRAK antibody (ab97332)
ab97332, at a 1/200 dilution, staining PRAK in paraformaldehyde fixed HeLa cells by Immunofluorescence.
Lower image is merged with DNA probe.
has not yet been referenced specifically in any publications.
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