• 产品名称Anti-PRAK抗体
    参阅全部 PRAK 一抗
  • 描述
    兔多克隆抗体to PRAK
  • 特异性ab79433 detects endogenous levels of total PRAK protein.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic non phosphopeptide derived from human PRAK around the phosphorylation site of threonine 182 (L-M-TP-P-Q).

  • 阳性对照
    • MCF7 cell extracts.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab79433 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab79433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ELISA 1/10000.


  • 功能Mediates stress-induced small heat shock protein 27 phosphorylation.
  • 组织特异性Expressed ubiquitously.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated on Thr-182; which is the regulatory phosphorylation site and is located on the T-loop/loop 12.
  • 细胞定位Cytoplasm. Nucleus. Also observed in the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MAP kinase-activated protein kinase 5 antibody
    • MAPK-activated protein kinase 5 antibody
    • MAPK5_HUMAN antibody
    • MAPKAP kinase 5 antibody
    • MAPKAPK-5 antibody
    • MAPKAPK5 antibody
    • Mitogen activated protein kinase activated protein kinase 5 antibody
    • p38-regulated/activated protein kinase antibody
    • PRAK antibody
    see all

Anti-PRAK antibody 图像

  • All lanes : Anti-PRAK antibody (ab79433) at 1/500 dilution

    Lane 1 : MCF7 cell extracts
    Lane 2 : MCF7 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 10 µg per lane.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa

Anti-PRAK antibody (ab79433)参考文献

ab79433 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (transformed murine B cells)
Loading amount 50 µg
Specification transformed murine B cells
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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提交于 Mar 05 2012

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We have not tested ab79433 in mouse tissue and only predict it will work based on sequence homology. As ab79433 has not been tested in mouse tissue, I can offer a discount off a future purcha...

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