概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to PRAF1
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human PRAF1 isoform 2 Swiss-Prot Q9GZS1 (NP_071935.1)

  • 阳性对照
    • U-2 OS cell lysate, PRAF1 transfected 293T cell lysate and HeLa cell

性能

应用

Our Abpromise guarantee covers the use of ab88623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.
  • 序列相似性
    Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.
  • 细胞定位
    Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A49 antibody
    • DNA directed RNA polymerase I subunit RPA49 antibody
    • DNA-directed RNA polymerase I subunit E antibody
    • DNA-directed RNA polymerase I subunit RPA49 antibody
    • FLJ 13390 antibody
    • FLJ13970 antibody
    • PAF53 antibody
    • POLR 1 E antibody
    • POLR1E antibody
    • Polymerase (RNA) I associated factor 1 antibody
    • Polymerase (RNA) I polypeptide E antibody
    • Polymerase (RNA) I polypeptide E, 53kDa antibody
    • PRAF1 antibody
    • RNA polymerase I associated factor 1 antibody
    • RNA polymerase I associated factor 53 antibody
    • RNA polymerase I polypeptide E antibody
    • RNA polymerase I subunit A49 antibody
    • RNA polymerase I-associated factor 1 antibody
    • RNA polymerase I-associated factor 53 antibody
    • RP11405L18.3 antibody
    • RPA49_HUMAN antibody
    see all

图片

  • Anti-PRAF1 antibody (ab88623) at 1 µg/ml + U-2 OS at 50 µg

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-PRAF1 antibody (ab88623) at 1 µg/ml

    Lane 1 : PRAF1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of ab88623 on HeLa cell (antibody concentration 10 ug/ml)

文献

ab88623 has not yet been referenced specifically in any publications.

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