Anti-PRAF1抗体(ab88623)
Key features and details
- Mouse polyclonal to PRAF1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PRAF1抗体 -
描述
小鼠多克隆抗体to PRAF1 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human PRAF1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- U-2 OS cell lysate, PRAF1 transfected 293T cell lysate and HeLa cell
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88623于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF. -
序列相似性
Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family. -
细胞定位
Nucleus > nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 64425 Human
- SwissProt: Q9GZS1 Human
- Unigene: 591087 Human
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别名
- A49 antibody
- DNA directed RNA polymerase I subunit RPA49 antibody
- DNA-directed RNA polymerase I subunit E antibody
see all
图片
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Anti-PRAF1 antibody (ab88623) at 1 µg/ml + U-2 OS at 50 µg
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-PRAF1 antibody (ab88623) at 1 µg/ml
Lane 1 : PRAF1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
Immunofluorescence of ab88623 on HeLa cell (antibody concentration 10 ug/ml)
实验方案
数据表及文件
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Datasheet download
文献 (0)
ab88623 尚未被引用在任何文献中。