Anti-PQBP1抗体(ab100797)
Key features and details
- Rabbit polyclonal to PQBP1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PQBP1抗体 -
描述
兔多克隆抗体to PQBP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide within Human PQBP1 aa 150-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_005701.1
Database link: O60828 -
阳性对照
- HeLa, 293T lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antibody was affinity purified using an epitope specific to PQBP1 immobilized on solid support -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab100797于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 30 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May suppress the ability of POU3F2 to transactivate the DRD1 gene in a POU3F2 dependent manner. Can activate transcription directly or via association with the transcription machinery. May be involved in ATXN1 mutant-induced cell death. The interaction with ATXN1 mutant reduces levels of phosphorylated RNA polymerase II large subunit. -
组织特异性
Widely expressed with high level in heart, skeletal muscle, pancreas, spleen, thymus, prostate, ovary, small intestine and peripheral blood leukocytes. -
疾病相关
Defects in PQBP1 are the cause of Renpenning syndrome 1 (RENS1) [MIM:309500]; also known as Sutherland-Haan X-linked mental retardation syndrome (SHS) or X-linked mental retardation syndromes MRXS3/MRXS8/MRX55. The clinical features are mental retardation, microcephaly, short stature, and small testes. The craniofacies tends to be narrow and tall with upslanting palpebral fissures, abnormal nasal configuration, cupped ears, and short philtrum. The nose may appear long or bulbous, with overhanging columella. Less consistent manifestations include ocular colobomas, cardiac malformations, cleft palate, and anal anomalies. RENS1 is more frequently in males than in females where little or no expression is found. -
序列相似性
Contains 1 WW domain. -
结构域
The WW domain may play a role as a transcriptional activator directly or via association with the transcription machinery. The WW domain mediates interaction with C-terminal domain of RNA polymerase II large subunit. -
细胞定位
Nucleus. Co-localized with POU3F2. Co-localized with ATXN1 in nuclear inclusion bodies. - Information by UniProt
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数据库链接
- Entrez Gene: 10084 Human
- Entrez Gene: 54633 Mouse
- Omim: 300463 Human
- SwissProt: O60828 Human
- SwissProt: Q91VJ5 Mouse
- Unigene: 534384 Human
- Unigene: 14616 Mouse
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别名
- 38 kDa nuclear protein containing a WW domain antibody
- Mental retardation, X linked 55 antibody
- MRX55 antibody
see all
图片
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All lanes : Anti-PQBP1 antibody (ab100797) at 0.1 µg/ml
Lane 1 : HeLa whole Cell Lysate at 50 µg
Lane 2 : HeLa whole Cell Lysate at 15 µg
Lane 3 : HeLa whole Cell Lysate at 5 µg
Lane 4 : 293T whole Cell Lysate at 50 µg
Predicted band size: 30 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling PQBP1 with ab100797 at 1/1000 (1µg/mg). Detection: DAB.
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ab100797 at 1 µg/ml staining PQBP1 in HeLa cell lysate immunoprecipitated using ab100797 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 10 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab100797 被引用在 1 文献中.
- Yang H et al. CPVL promotes glioma progression via STAT1 pathway inhibition through interactions with the BTK/p300 axis. JCI Insight 6:N/A (2021). PubMed: 34784299