概述

  • 产品名称Anti-PPT1抗体
    参阅全部 PPT1 一抗
  • 描述
    小鼠多克隆抗体to PPT1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human PPT1, amino acids 1-306 (NP_000301.1)

  • 阳性对照
    • Human liver tissue lysate, PPT1 transfected 293T cell lysate, Human salivary gland tissue.

性能

应用

Our Abpromise guarantee covers the use of ab89022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is recommended Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
    • 疾病相关Defects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1 (CLN1) [MIM:256730]. A form of neuronal ceroid lipofuscinosis with variable age at onset. Infantile, late-infantile, juvenile, and adult onset have been reported. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN1 is referred to as granular osmiophilic deposits (GROD).
    • 序列相似性Belongs to the palmitoyl-protein thioesterase family.
    • 细胞定位Lysosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Ceroid palmitoyl palmitoyl protein thioesterase 1 antibody
      • CLN1 antibody
      • EC 3.1.2.22 antibody
      • INCL antibody
      • Palmitoyl protein hydrolase 1 antibody
      • Palmitoyl protein thioesterase 1 antibody
      • Palmitoyl-protein hydrolase 1 antibody
      • Palmitoyl-protein thioesterase 1 antibody
      • PPT antibody
      • PPT-1 antibody
      • PPT1 antibody
      • PPT1_HUMAN antibody
      see all

    Anti-PPT1 antibody 图像

    • Anti-PPT1 antibody (ab89022) at 1 µg/ml + Human liver tissue lysate at 50 µg

      Predicted band size : 34 kDa
      Observed band size : 34 kDa
    • All lanes : Anti-PPT1 antibody (ab89022) at 1 µg/ml

      Lane 1 : PPT1 transfected 293T cell lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 34 kDa
      Observed band size : 34 kDa
    • ab89022 at 3µg/ml staining PPT1 in Human salivary gland by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

    Anti-PPT1 antibody (ab89022)参考文献

    ab89022 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab89022.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"