Anti-PPT1/PPT抗体(ab89022)
Key features and details
- Mouse polyclonal to PPT1/PPT
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-PPT1/PPT抗体
参阅全部 PPT1/PPT 一抗 -
描述
小鼠多克隆抗体to PPT1/PPT -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human PPT1/PPT. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_000301.1 -
阳性对照
- WB: Human liver tissue lysate, PPT1/PPT transfected HEK-293T cell lysate. IHC-P: Human salivary gland tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab89022于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | ||
IHC-P |
说明 |
---|
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
-
功能
Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons. -
疾病相关
Defects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1 (CLN1) [MIM:256730]. A form of neuronal ceroid lipofuscinosis with variable age at onset. Infantile, late-infantile, juvenile, and adult onset have been reported. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN1 is referred to as granular osmiophilic deposits (GROD). -
序列相似性
Belongs to the palmitoyl-protein thioesterase family. -
细胞定位
Lysosome. - Information by UniProt
-
数据库链接
- Entrez Gene: 5538 Human
- Omim: 600722 Human
- SwissProt: P50897 Human
- Unigene: 3873 Human
-
别名
- Ceroid palmitoyl palmitoyl protein thioesterase 1 antibody
- CLN1 antibody
- EC 3.1.2.22 antibody
see all
图片
-
Anti-PPT1/PPT antibody (ab89022) at 1 µg/ml + Human liver tissue lysate at 50 µg
Predicted band size: 34 kDa
Observed band size: 34 kDa -
All lanes : Anti-PPT1/PPT antibody (ab89022) at 1 µg/ml
Lane 1 : PPT1/PPT transfected HEK-293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa -
ab89022 at 3µg/ml staining PPT1/PPT in Human salivary gland by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
数据表及文件
-
Datasheet download
文献 (3)
ab89022 被引用在 3 文献中.
- Sima N et al. Neural stem cells for disease modeling and evaluation of therapeutics for infantile (CLN1/PPT1) and late infantile (CLN2/TPP1) neuronal ceroid lipofuscinoses. Orphanet J Rare Dis 13:54 (2018). WB . PubMed: 29631617
- Shyng C et al. Widespread Expression of a Membrane-Tethered Version of the Soluble Lysosomal Enzyme Palmitoyl Protein Thioesterase-1. JIMD Rep 36:85-92 (2017). PubMed: 28213849
- Wang R et al. Identification of palmitoyl protein thioesterase 1 in human THP1 monocytes and macrophages and characterization of unique biochemical activities for this enzyme. Biochemistry 52:7559-74 (2013). PubMed: 24083319