• 产品名称Anti-PPT1抗体
    参阅全部 PPT1 一抗
  • 描述
    兔多克隆抗体to PPT1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH-conjugated synthetic peptide (10-30 aa in length) at the C-term of last 50 aa of human PPT1.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab38417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 34 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.


  • 功能Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
  • 疾病相关Defects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1 (CLN1) [MIM:256730]. A form of neuronal ceroid lipofuscinosis with variable age at onset. Infantile, late-infantile, juvenile, and adult onset have been reported. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN1 is referred to as granular osmiophilic deposits (GROD).
  • 序列相似性Belongs to the palmitoyl-protein thioesterase family.
  • 细胞定位Lysosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ceroid palmitoyl palmitoyl protein thioesterase 1 antibody
    • CLN1 antibody
    • EC antibody
    • INCL antibody
    • Palmitoyl protein hydrolase 1 antibody
    • Palmitoyl protein thioesterase 1 antibody
    • Palmitoyl-protein hydrolase 1 antibody
    • Palmitoyl-protein thioesterase 1 antibody
    • PPT antibody
    • PPT-1 antibody
    • PPT1 antibody
    • PPT1_HUMAN antibody
    see all

Anti-PPT1 antibody 图像

  • Anti-PPT1 antibody (ab38417) at 1/100 dilution + 293 cell lysate.

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-PPT1 antibody (ab38417)参考文献

ab38417 has not yet been referenced specifically in any publications.

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Thank you for your reply.

As requested, I have issued a free of charge replacement of ab89022.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this fr...

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Thank you for your reply and for trying out my suggestions.

I think you are making some progress diluting out the secondary antibody. The background is way too high for a secondary antibody and to properly assess the results. I do notice ...

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Thank you for your reply.

I appreciate that you tested the suggestions made to improve your results. It is very alarming how much background you observe in the absence of primary. As a next step, it is necessary to further dilute the seco...

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Es freut mich sehr zu hören, dass ab38417 nun guteErgebnisse liefertund wünsche Ihnen viel Erfolg mit Ihrer Forschung.

Thank you for your reply.

I would recommend that you also test some higher dilutions of antibody (test 1:250 and 1:500) to help eliminate some background bands. Additionally, I would recommend using 5% BSA rather than milk to help reduce ...

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Thank you for your reply with this information.

The results of your WB with the human liver tissue are very promising, likely reflective of the band of interest as well as some glycosylated protein. What dilutions of antibody did you test...

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Thank you for contacting Abcam regarding ab38417.

I am sorry that you have been experiencing difficulties with this antibody in WB. In order to better understand the problems you are having in WB I would like to get some additional information...

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