Anti-PPRC1抗体(ab80900)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to PPRC1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 700 - 800 of Human PPRC1.

    (Peptide available as ab90210.)

  • 阳性对照
    • This antibody gave a positive signal in Human Liver Tissue Lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 178 kDa).

靶标

  • 相关性
    PPRC1 acts as a coactivator during transcriptional activation of nuclear genes related to mitochondrial biogenesis and cell growth. It is involved in the transcription coactivation of CREB and NRF1 target genes.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • Peroxisome proliferator-activated receptor gamma coactivator-related protein 1 antibody
    • PGC-1-related coactivator antibody
    • PRC antibody

图片

  • Anti-PPRC1 antibody (ab80900) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 178 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

实验方案

文献

ab80900 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

提交于 May 31 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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