Anti-PPP6C抗体(ab70634)

概述

  • 产品名称Anti-PPP6C抗体
    参阅全部 PPP6C 一抗
  • 描述
    兔多克隆抗体to PPP6C
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to a region between residue 250 and the C-terminus (residue 305) of Human PPP6C (NP_002712.1)

  • 阳性对照
    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

Anti-PPP6C antibody 图像

  • All lanes : Anti-PPP6C antibody (ab70634) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 35 kDa
    Observed band size : 35 kDa


    Exposure time : 3 minutes
  • Detection of PPP6C by Western Blot of Immunoprecipitate. ab70634 at 1µg/ml staining PPP6C in HeLa whole cell lysate immunoprecipitated using ab70634 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 30 seconds.

Anti-PPP6C antibody (ab70634)参考文献

ab70634 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (fibroblast)
Loading amount 25 µg
Specification fibroblast
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Aug 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"