Anti-PPP6C抗体(ab60249)

Submit an Abreview Q&A (2)

概述

  • 产品名称
    Anti-PPP6C抗体
    参阅全部 PPP6C 一抗
  • 描述
    兔多克隆抗体to PPP6C
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the N-terminal region of human PPP6C.

  • 阳性对照
    • Thymus cell lysate or human breast carcinoma tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab60249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 功能
    Catalytic subunit of protein phospatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7.
  • 组织特异性
    Ubiquitously expressed in all tissues tested with highest expression levels in testis, heart, kidney, brain, stomach, liver and skeletal muscle and lowest in placenta, lung colon and spleen.
  • 序列相似性
    Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
    • 别名
      • FLJ92648 antibody
      • MGC12249 antibody
      • PP 6 antibody
      • PP6 antibody
      • PP6C antibody
      • PPP 6 antibody
      • PPP 6C antibody
      • PPP6 antibody
      • PPP6_HUMAN antibody
      • Ppp6c antibody
      • Protein phosphatase 6 catalytic subunit antibody
      • Serine/threonine protein phosphatase 6 antibody
      • Serine/threonine protein phosphatase catalytic subunit antibody
      • Serine/threonine-protein phosphatase 6 catalytic subunit antibody
      see all

    图片

    • Western blot - Anti-PPP6C antibody (ab60249)
      Western blot - Anti-PPP6C antibody (ab60249)
      Anti-PPP6C antibody (ab60249) at 1/100 dilution + Thymus cell lysate

      Predicted band size: 35 kDa
      Observed band size: 37 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP6C antibody (ab60249)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP6C antibody (ab60249)
      Ab60249 (1/50-1/100) staining human PPP6C in human breast carcinoma tissue by immunohsitochemistry using formalin fixed, paraffin embedded tissue.

      Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

      BC = breast carcinoma

    文献

    ab60249 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab60249？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    2 reviews or Q&A

    10
    Votes: Highest First

    will test either ab70634 or ab60249 in Arabidopsis

    Thank you for contacting us.

    Please let me know which antibody you would like to try inArabidopsis (ab70634 or ab60249), so that I can send you the testing discount code.

    I look forward to hear back from you.

    Use our pro...

    Read More

    I have a question about an antibody cross-species specificity. I found antibodies against human PP6 in your catalog: ab60249 and ab70634. I am wondering whether these antibodies are able to recognize Drosophila (fruit fly) PP6 protein. Do you have any ...

    Read More

    Thank you for contacting Abcam. I have confirmed with the laboratory that neither ab60249 nor ab70634 share significant homology with the Drosophila sequence.  We do not expect these antibodies to cross react with this species. I apologize for ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册