Anti-PPP6C抗体(ab60249)

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概述

  • 产品名称
    Anti-PPP6C抗体
    参阅全部 PPP6C 一抗
  • 描述
    兔多克隆抗体to PPP6C
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the N-terminal region of human PPP6C.

  • 阳性对照
    • Thymus cell lysate or human breast carcinoma tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab60249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 功能
    Catalytic subunit of protein phospatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7.
  • 组织特异性
    Ubiquitously expressed in all tissues tested with highest expression levels in testis, heart, kidney, brain, stomach, liver and skeletal muscle and lowest in placenta, lung colon and spleen.
  • 序列相似性
    Belongs to the PPP phosphatase family. PP-6 (PP-V) subfamily.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
    • 别名
      • FLJ92648 antibody
      • MGC12249 antibody
      • PP 6 antibody
      • PP6 antibody
      • PP6C antibody
      • PPP 6 antibody
      • PPP 6C antibody
      • PPP6 antibody
      • PPP6_HUMAN antibody
      • Ppp6c antibody
      • Protein phosphatase 6 catalytic subunit antibody
      • Serine/threonine protein phosphatase 6 antibody
      • Serine/threonine protein phosphatase catalytic subunit antibody
      • Serine/threonine-protein phosphatase 6 catalytic subunit antibody
      see all

    Anti-PPP6C antibody 图像

    • Western blot - PPP6C antibody (ab60249)
      Western blot - PPP6C antibody (ab60249)
      Anti-PPP6C antibody (ab60249) at 1/100 dilution + Thymus cell lysate

      Predicted band size : 35 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PPP6C antibody (ab60249)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PPP6C antibody (ab60249)
      Ab60249 (1/50-1/100) staining human PPP6C in human breast carcinoma tissue by immunohsitochemistry using formalin fixed, paraffin embedded tissue.

      Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

      BC = breast carcinoma

    Anti-PPP6C antibody (ab60249)参考文献

    ab60249 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab60249？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    will test either ab70634 or ab60249 in Arabidopsis

    Thank you for contacting us.

    Please let me know which antibody you would like to try inArabidopsis (ab70634 or ab60249), so that I can send you the testing discount code.

    I look forward to hear back from you.

    Use our pro...

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    I have a question about an antibody cross-species specificity. I found antibodies against human PP6 in your catalog: ab60249 and ab70634. I am wondering whether these antibodies are able to recognize Drosophila (fruit fly) PP6 protein. Do you have any ...

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    Thank you for contacting Abcam. I have confirmed with the laboratory that neither ab60249 nor ab70634 share significant homology with the Drosophila sequence.  We do not expect these antibodies to cross react with this species. I apologize for ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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