概述

  • 产品名称Anti-PPP4R2抗体
    参阅全部 PPP4R2 一抗
  • 描述
    兔多克隆抗体to PPP4R2
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 370 and the C-terminus (residue 417) of Human PPP4R2 (NP_777567.1)

  • 阳性对照
    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab70631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 47 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-PPP4R2 antibody 图像

    • All lanes : Anti-PPP4R2 antibody (ab70631) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 47 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds
    • Detection of Human PPP4R2 by Western Blot of Immunprecipitate. ab70631 at 1µg/ml staining PPP4R2 in HeLa whole cell lysate immunoprecipitated using ab70631 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: chemiluminescence with exposure time of 10 seconds.

    Anti-PPP4R2 antibody (ab70631)参考文献

    This product has been referenced in:

    See 1 Publication for this product

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