概述
- 产品名称Anti-PPP4C抗体
参阅全部 PPP4C 一抗 - 描述山羊多克隆抗体to PPP4C
- 经测试应用适用于: WB, IHC-Pmore details
- 种属反应性与反应: Human
预测可用于: Mouse, Rat, Rabbit, Cow, Dog, Xenopus laevis, Zebrafish, Orangutan
- 免疫原
Synthetic peptide:
C-PQETRGIPSKKP
, corresponding to C terminal amino acids 290-301 of Human PPP4C (NP_002711.1). - 阳性对照
- HEK293, A431 and HeLa cell lysates.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 -
Concentration information loading... - 纯度Immunogen affinity purified
- 纯化说明ab87672 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
- Positive Controls
应用
Our Abpromise guarantee covers the use of ab87672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). | |
| IHC-P | Use a concentration of 2 - 4 µg/ml. |
靶标
- 功能Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
- 序列相似性Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.
- 细胞定位Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
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数据库链接
- Entrez Gene: 540398 Cow
- Entrez Gene: 5531 Human
- Entrez Gene: 56420 Mouse
- Entrez Gene: 100173513 Orangutan
- Entrez Gene: 100009163 Rabbit
- Entrez Gene: 171366 Rat
- Entrez Gene: 432334 Xenopus laevis
- Entrez Gene: 100003080 Zebrafish
see all -
别名
- PP X antibody
- PP-X antibody
- Pp4 antibody
see all
Anti-PPP4C antibody 图像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP4C antibody (ab87672)Immunohistochemical staining of paraffin embedded human prostate with ab87672 at 2.5 µg/mL. Heat mediated antigen retrieval was performed with citrate buffer at pH 6.
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Western blot - Anti-PPP4C antibody (ab87672)All lanes : Anti-PPP4C antibody (ab87672) at 0.3 µg/ml
Lane 1 : rat spleen tissue lysate
Lane 2 : rat lung tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size : 34 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?) -
Western blot - PPP4C antibody (ab87672)Anti-PPP4C antibody (ab87672) at 0.3 µg/ml + HEK293 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique
Predicted band size : 34 kDa
Observed band size : 34 kDa
Primary incubation was 1 hour.
Anti-PPP4C antibody (ab87672)参考文献
ab87672 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"