概述

  • 产品名称Anti-PPP4C抗体
    参阅全部 PPP4C 一抗
  • 描述
    兔多克隆抗体to PPP4C
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1 and 50 of human PPP4C (NP_002711.1).

  • 阳性对照
    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IP Use at 2-4 µg/mg of lysate.

靶标

  • 功能Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
  • 序列相似性Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • PP X antibody
    • PP-X antibody
    • Pp4 antibody
    • PP4C antibody
    • PP4C_HUMAN antibody
    • PPH3 antibody
    • PPP4 antibody
    • ppp4c antibody
    • PPX antibody
    • protein phosphatase 4 (formerly X), catalytic subunit antibody
    • Protein phosphatase 4 catalytic subunit antibody
    • Protein phosphatase X antibody
    • protein phosphatase X, catalytic subunit antibody
    • Serine/threonine protein phosphatase 4 catalytic subunit antibody
    • Serine/threonine-protein phosphatase 4 catalytic subunit antibody
    see all

Anti-PPP4C antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PPP4C with ab70623 at 0.2µg/ml (left) and 1µg/ml (right). Detection: DAB.
  • All lanes : Anti-PPP4C antibody (ab70623) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 250 kDa,28 kDa,80 kDa. We are unsure as to the identity of these extra bands.

Anti-PPP4C antibody (ab70623)参考文献

ab70623 has not yet been referenced specifically in any publications.

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