Anti-PPP4C抗体(ab16475)

概述

  • 产品名称Anti-PPP4C抗体
    参阅全部 PPP4C 一抗
  • 描述
    兔多克隆抗体to PPP4C
  • 经测试应用适用于: ICC/IF, WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
    预测可用于: Rabbit
  • 免疫原

    Synthetic peptide:

    RGIPSKKPVADYFL

    conjugated to KLH, corresponding to amino acids 294-307 of Human PPP4C.

  • 阳性对照
    • Bovine testes homogenate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab16475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/300.
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
ICC 1/500 - 1/1000.

靶标

  • 功能Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
  • 序列相似性Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • PP X antibody
    • PP-X antibody
    • Pp4 antibody
    • PP4C antibody
    • PP4C_HUMAN antibody
    • PPH3 antibody
    • PPP4 antibody
    • ppp4c antibody
    • PPX antibody
    • protein phosphatase 4 (formerly X), catalytic subunit antibody
    • Protein phosphatase 4 catalytic subunit antibody
    • Protein phosphatase X antibody
    • protein phosphatase X, catalytic subunit antibody
    • Serine/threonine protein phosphatase 4 catalytic subunit antibody
    • Serine/threonine-protein phosphatase 4 catalytic subunit antibody
    see all

Anti-PPP4C antibody 图像

  • All lanes : Anti-PPP4C antibody (ab16475) at 1/10000 dilution (in PBS + 5% milk +0.1% Tween 20 for 1 hour at 25°C)

    Lane 1 : HeLa whole cell lysate - mock transfected
    Lane 2 : HeLa whole cell lysate - transfected with human siRNA against PPP4C

    Lysates/proteins at 100000 cells per lane.

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/50000 dilution

    Performed under non-reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    This image is courtesy of an anonymous abreview.

    Blocking Step: 5% Milk for 1 hour at 25°C

    See Abreview

Anti-PPP4C antibody (ab16475)参考文献

ab16475 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10000 cells
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Sample Human Cell lysate - whole cell (Huh7 liver)
Specification Huh7 liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 31 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela cells)
Loading amount 100000 cells
Specification Hela cells
Gel Running Conditions Non-reduced Non-Denaturing (Native) (Invitrogen NuPage 4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela)
Specification Hela
Fixative Methanol
Permeabilization No
Blocking step Fish scale gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 15 2010

I think there are two potential areas which the customer should consider looking into: the protocol and the samples. I am sure the customer knows very well his area of research and trust that many papers show PPP4C where he expects it, unfortunately, ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"