概述

  • 产品名称Anti-PPP2R5D抗体
    参阅全部 PPP2R5D 一抗
  • 描述
    小鼠单克隆抗体to PPP2R5D
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Pig
  • 免疫原

    Recombinant fragment: RLNPQYPMFR APPPLPPVYS METETPTAED IQLLKRTVET EAVQMLKDIK KEKVLLRRKS ELPQDVYTIK ALEAHKRAEE FLTASQEAL, corresponding to amino acids 514-603 of Human PPP2R5D, with a tag of 26kDa. NP_006236.

  • 阳性对照
    • Recombinant protein and Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab88075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 67 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • 组织特异性Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.
  • 序列相似性Belongs to the phosphatase 2A regulatory subunit B56 family.
  • 细胞定位Cytoplasm. Nucleus. Nuclear in interphase, nuclear during mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2A5D_HUMAN antibody
    • B'delta antibody
    • B56D antibody
    • Delta isoform of regulatory subunit B56, protein phosphatase 2A antibody
    • MGC2134 antibody
    • MGC8949 antibody
    • OTTHUMP00000039821 antibody
    • PP2A B subunit B' delta isoform antibody
    • PP2A B subunit B56 delta isoform antibody
    • PP2A B subunit isoform B''-delta antibody
    • PP2A B subunit isoform B56-delta antibody
    • PP2A B subunit isoform PR61-delta antibody
    • PP2A B subunit isoform R5-delta antibody
    • PP2A B subunit PR61 delta isoform antibody
    • PP2A B subunit R5 delta isoform antibody
    • PPP2R5D antibody
    • Protein phosphatase 2 regulatory subunit B (B56) delta isoform antibody
    • Protein phosphatase 2 regulatory subunit B delta isoform antibody
    • Protein phosphatase 2 regulatory subunit B' delta antibody
    • Serine threonine protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody
    • TEG-271 antibody
    • Tex271 antibody
    see all

Anti-PPP2R5D antibody 图像

  • Anti-PPP2R5D antibody (ab88075) at 5 µg/ml + recombinant protein (immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 67 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Anti-PPP2R5D antibody (ab88075) at 1/500 dilution + Jurkat cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 67 kDa
    Observed band size : 67 kDa

Anti-PPP2R5D antibody (ab88075)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HeLa nuclear cell lysate)
Loading amount 25 µg
Specification HeLa nuclear cell lysate
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 May 15 2013

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing (10)
Sample Human Cell lysate - whole cell (CCRF-CEM)
Specification CCRF-CEM
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 Jan 19 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step 5% BSA + 1% NDS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Jul 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"