概述

  • 产品名称
    Anti-PPP2R5D抗体
    参阅全部 PPP2R5D 一抗
  • 描述
    小鼠单克隆抗体to PPP2R5D
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Pig
  • 免疫原

    Recombinant fragment: RLNPQYPMFR APPPLPPVYS METETPTAED IQLLKRTVET EAVQMLKDIK KEKVLLRRKS ELPQDVYTIK ALEAHKRAEE FLTASQEAL, corresponding to amino acids 514-603 of Human PPP2R5D, with a tag of 26kDa. NP_006236.

  • 阳性对照
    • Recombinant protein and Jurkat cell lysate.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

应用

Our Abpromise guarantee covers the use of ab88075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 67 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
  • 组织特异性
    Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.
  • 序列相似性
    Belongs to the phosphatase 2A regulatory subunit B56 family.
  • 细胞定位
    Cytoplasm. Nucleus. Nuclear in interphase, nuclear during mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2A5D_HUMAN antibody
    • B'delta antibody
    • B56D antibody
    • Delta isoform of regulatory subunit B56, protein phosphatase 2A antibody
    • MGC2134 antibody
    • MGC8949 antibody
    • OTTHUMP00000039821 antibody
    • PP2A B subunit B' delta isoform antibody
    • PP2A B subunit B56 delta isoform antibody
    • PP2A B subunit isoform B''-delta antibody
    • PP2A B subunit isoform B56-delta antibody
    • PP2A B subunit isoform PR61-delta antibody
    • PP2A B subunit isoform R5-delta antibody
    • PP2A B subunit PR61 delta isoform antibody
    • PP2A B subunit R5 delta isoform antibody
    • PPP2R5D antibody
    • Protein phosphatase 2 regulatory subunit B (B56) delta isoform antibody
    • Protein phosphatase 2 regulatory subunit B delta isoform antibody
    • Protein phosphatase 2 regulatory subunit B' delta antibody
    • Serine threonine protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody
    • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody
    • TEG-271 antibody
    • Tex271 antibody
    see all

Anti-PPP2R5D antibody 图像

  • Anti-PPP2R5D antibody (ab88075) at 5 µg/ml + recombinant protein (immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 67 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Anti-PPP2R5D antibody (ab88075) at 1/500 dilution + Jurkat cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 67 kDa
    Observed band size : 67 kDa

Anti-PPP2R5D antibody (ab88075)参考文献

This product has been referenced in:
  • Greenwood EJ  et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. Elife 5:N/A (2016). Read more (PubMed: 27690223) »
  • Porter IM  et al. Bod1 regulates protein phosphatase 2A at mitotic kinetochores. Nat Commun 4:2677 (2013). WB ; Human . Read more (PubMed: 24157919) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (HeLa nuclear cell lysate)
Loading amount
25 µg
Specification
HeLa nuclear cell lysate
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 May 15 2013

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (CCRF-CEM)
Specification
CCRF-CEM
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jan 19 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
5% BSA + 1% NDS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册