概述

性能

应用

Our Abpromise guarantee covers the use of ab1366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    ELISA: Detection limit dilution 1/2000.
    WB: Use at a concentration of 2 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 on the 'Thr-308' and 'Ser-373' residues.
    • 组织特异性
      Highest expression in brain.
    • 序列相似性
      Belongs to the phosphatase 2A regulatory subunit B56 family.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2A5B_HUMAN antibody
      • B56B antibody
      • Beta isoform of regulatory subunit B56 protein phosphatase 2A antibody
      • FLJ35411 antibody
      • PP2A B subunit B' beta isoform antibody
      • PP2A B subunit B56 beta isoform antibody
      • PP2A B subunit isoform B' beta antibody
      • PP2A B subunit isoform B''-beta antibody
      • PP2A B subunit isoform B56-beta antibody
      • PP2A B subunit isoform PR61-beta antibody
      • PP2A B subunit isoform R5-beta antibody
      • PP2A B subunit PR61 beta isoform antibody
      • PP2A B subunit R5 beta isoform antibody
      • PPP2R5B antibody
      • PR 61B antibody
      • PR61B antibody
      • Protein phosphatase 2 regulatory subunit B (B56) beta antibody
      • Protein phosphatase 2 regulatory subunit B (B56) beta isoform antibody
      • Protein phosphatase 2 regulatory subunit B' beta antibody
      • Protein phosphatase 2 regulatory subunit B' beta isoform antibody
      • Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit beta isoform antibody
      • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform antibody
      see all

    Anti-PPP2R5B antibody 图像

    • Anti-PPP2R5B antibody (ab1366) at 2 µg/ml + Human cerebellum lysate at 35 µg

      Predicted band size : 55 kDa
      Observed band size : 55 kDa

    实验方案

    Anti-PPP2R5B antibody (ab1366)参考文献

    This product has been referenced in:
    • DeGrande ST  et al. Molecular mechanisms underlying cardiac protein phosphatase 2A regulation in heart. J Biol Chem 288:1032-46 (2013). WB ; Human, Mouse, Rat, Dog . Read more (PubMed: 23204520) »

    See 1 Publication for this product

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293T)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    HEK293T
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Mar 31 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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